SCC226
YUMM1.4 Mouse Melanoma Cell Line
Synonyme(s) :
YUMM1.4 Mouse Melanoma Cell Line, Yale University Mouse Melanoma 1.4 cell line, Yale University Mouse Melanoma cell line
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About This Item
Code UNSPSC :
41106514
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conditionnement
vial of ≥1 x 10^6 vial (viable cells per vial)
Fabricant/nom de marque
Millipore
Technique(s)
cell culture | mammalian: suitable
Conditions d'expédition
liquid nitrogen
Température de stockage
−196°C
Application
- Each vial contains > 1X106 viable cells.
- YUMM1.4 cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
- Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
- Cells are negative for mycoplasma contamination.
Caractéristiques et avantages
The YUMM1.4 cell line is driven by Braf activation, Pten inactivation, and Cdkn2a inactivation. The cell line is positive for the common melanoma marker, MelanA. The cells are syngeneic with C57BL/6J mice and are very tumorigenic in vivo.
Description de la cible
As immune-based therapies in cancer models become more popular and applicable, a further need for immune-competent models has been presented. A wide variety of genetically engineered mouse models have been created to express vital melanoma genotypes. The variety in these models have allowed the study of genetic alterations that are relevant to human melanoma biology. While it is quite useful to have mouse models with genetically defined human melanoma characteristics, it is limited by complex mouse colony maintenance.
The YUMM1.4 cell line was produced to be more experimentally relevant. The cell line was derived by backcrossing important alleles into C57BL/6J mice. The mice were interbred to produce human-relevant genetically defined mouse models that could then be used to generate cell lines. Melanomas were induced by applying 4-hydroxytamoxifen to induce Cre-lox allele recombination. Cells were then mechanically and enzymatically dissociated from the removed tumor. The melanoma lines generated from this procedure retained important human melanoma characteristics useful for studying disease progression.
The YUMM1.4 cell line is driven by Braf activation, Pten inactivation, and Cdkn2a inactivation.1 The cell line is positive for the common melanoma marker, MelanA. The cells are syngeneic with C57BL/6J mice and are very tumorigenic in vivo.
Source
Cell line was derived from a 4-hydroxytamoxifen-induced melanoma tumor in a male C57BL/6 mouse into which mutations from the Braf/Pten genetically-engineered mouse model had been introduced via backcrossing.
References
1. Meeth, K., Wang, J. X., Micevic, G., Damsky, W., & Bosenberg, M. W. (2016). The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations.Pigment cell & melanoma research,29(5), 590-597.
The YUMM1.4 cell line was produced to be more experimentally relevant. The cell line was derived by backcrossing important alleles into C57BL/6J mice. The mice were interbred to produce human-relevant genetically defined mouse models that could then be used to generate cell lines. Melanomas were induced by applying 4-hydroxytamoxifen to induce Cre-lox allele recombination. Cells were then mechanically and enzymatically dissociated from the removed tumor. The melanoma lines generated from this procedure retained important human melanoma characteristics useful for studying disease progression.
The YUMM1.4 cell line is driven by Braf activation, Pten inactivation, and Cdkn2a inactivation.1 The cell line is positive for the common melanoma marker, MelanA. The cells are syngeneic with C57BL/6J mice and are very tumorigenic in vivo.
Source
Cell line was derived from a 4-hydroxytamoxifen-induced melanoma tumor in a male C57BL/6 mouse into which mutations from the Braf/Pten genetically-engineered mouse model had been introduced via backcrossing.
References
1. Meeth, K., Wang, J. X., Micevic, G., Damsky, W., & Bosenberg, M. W. (2016). The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations.Pigment cell & melanoma research,29(5), 590-597.
Stockage et stabilité
YUMM1.4 cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Autres remarques
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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