N27 Rat Dopaminergic Neural Cell Line, The N27 rat dopaminergic neural cell line has been used as a dopaminergic neuron model for in vitro and in vivo studies.
Parkinson’s disease is a neurodegenerative disorder of the central nervous system that affects more than 6 million people worldwide. The motor symptoms of Parkinson′s disease result from the death of dopamine generating cells in the substantia nigra, a region of the midbrain. Numerous efforts have been made to slow the loss or replace these dopamine producing neurons in vivo.
The N27 rat dopaminergic neuron cell line was harvested from E12 rat mesencephalic tissue and was transfected with SV40 to immortalize the cell line. The N27 cell line, when injected into the striata of 6-hydroxydopamine-lesioned rats (an animal model of PD) caused a time-dependent improvement in neurological deficits. This immortalized cell line has been carefully characterized in studies of dopamine biosynthesis, neurotoxicity and used as a dopaminergic neuron model for in vitro and in vivo studies.
Description de la lignée cellulaire
Neural Stem Cells
Neural Lineage Cells
Application
Research Category Stem Cell Research
Neuroscience
Research Sub Category Neural Stem Cells
Neurochemistry & Neurotrophins
Neurodegenerative Diseases
Neuroregenerative Medicine
This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact [email protected].
Qualité
• Each vial contains ≥ 1X106 viable cells. • Cells are negative for mycoplasma contamination.
Stockage et stabilité
N27 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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