This product does contain Lactate. Please see the full product formulation below. Quantities are in mg/L.
MR-121
EmbryoMax® KSOM Mouse Embryo Media
(1X), Liquid, with 1/2 Amino Acids & Phenol Red
Synonyme(s) :
K+ Simplex Optimised Medium (KSOM), KSOM Media
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50 ML
63,70 €
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Changer de vue
50 ML
63,70 €
About This Item
Code UNSPSC :
12352207
eCl@ss :
32160801
Nomenclature NACRES :
NA.75
Produits recommandés
Niveau de qualité
Forme
liquid
Fabricant/nom de marque
Specialty Media
EmbryoMax®
Technique(s)
cell culture | embryo: suitable
cell culture | stem cell: suitable
Entrée
sample type: mouse embryo(s)
Application
Stockage et stabilité
Can be stored at -20°C upto the expiration date on the bottle.
Once thawed it should be used within 2 weeks
Once thawed it should be used within 2 weeks
Informations légales
EmbryoMax is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Les clients ont également consulté
Chao-Lien Liu et al.
The Journal of biological chemistry, 282(2), 1109-1118 (2006-11-17)
l(2)dtl (lethal (2) denticleless), is an embryonic lethal homozygous mutation initially identified in Drosophila melanogaster that produces embryos that lack ventral denticle belts. In addition to nucleotide sequence, bioinformatic analysis has revealed a conservation of critical functional motifs among the
Christiana Kyvelidou et al.
Journal of structural biology, 176(3), 379-386 (2011-10-04)
Embryo patterning is subject to intense investigation. So far only large, microscopically obvious structures like polar body, cleavage furrow, pro-nucleus shape can be evaluated in the intact embryo. Using non-linear microscopic techniques, the present work describes new methodologies to evaluate
A potential use of embryonic stem cell medium for the in vitro culture of preimplantation embryos.
Gelber K. et al.
Journal of Assisted Reproduction and Genetics null
Hiroyuki Hirai et al.
Stem cells (Dayton, Ohio), 29(9), 1349-1361 (2011-07-07)
Induced pluripotent stem cells (iPSCs) can be created by reprogramming differentiated cells through introduction of defined genes, most commonly Oct4, Sox2, Klf4, and c-Myc (OSKM). However, this process is slow and extremely inefficient. Here, we demonstrate radical acceleration of iPSC
Marina Gertsenstein et al.
PloS one, 5(6), e11260-e11260 (2010-06-29)
Genetically modified mouse strains derived from embryonic stem (ES) cells have become essential tools for functional genomics and biomedical research. Large scale mutagenesis projects are producing libraries of mutant C57BL/6 (B6) ES cells to enable the functional annotation of every
Articles
Advanced KSOM mouse embryo media that can be used as a single medium for both harvest and culture of mouse embryos to facilitate creation of transgenic knockout mice.
Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture
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HI, DOES IT HAVE ANY LACTATE IN THE MEDIUM? IF SO, WHAT CONCENTRATION?
1 réponse -
Is the KSOM (MR-121-D) sufficient for embryo culture to the blastocyst stage on its own? In the literature, papers mention using "KSOM supplemented with amino acids." I observed that MR-121-D indicates it has "half amino acids." Essentially, I am trying to understand if individuals are adding extra amino acids to this media, or if the 1/2 amino acids already included are what they are referring to when they say "supplemented with amino acids."
1 réponse-
KSOM (MR-121-D) is indeed sufficient for embryo culture up to the blastocyst stage. It is a 1X liquid solution with 1/2 amino acids and Phenol Red. When scientists reference KSOM with 1/2 amino acids, that is the intended composition, and they do not add the remaining 1/2 amino acids.
For more detailed information and historical context, the article "A brief history of the development of the KSOM family of media" provides insights into the development and usage of KSOM. According to documented literature and the mentioned article, KSOM has been shown to support a high yield of mouse blastocysts capable of developing into fetuses after transfer to surrogate mothers. The medium was notably improved when supplemented with 19 natural amino acids, with the added amino acids present at half the concentration described by Eagle. Dose response studies indicated little difference in the response between full strength and half strength concentrations of the amino acids.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3790120/
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