MABT914

Anti-CD9 Antibody, clone Z9.1

clone Z9.1, from mouse

• Synonyme(s) : 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, MRP-1, Tetraspanin-29, Tspan-29, p24)
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: Z9.1, monoclonal
• Espèces réactives: human
• Technique(s): ELISA: suitable; flow cytometry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_001760
• Numéro d'accès UniProt: P21926
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CD9(928)

Description

Description générale

CD9 antigen (UniProt: P21926; also known as 5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; MRP-1; Tetraspanin-29; Tspan-29; p24) is encoded by the CD9 (also known as MIC3, TSPAN29; IG2) gene (Gene ID: 928) in human. CD9 is a multi-pass membrane glycoprotein of the tetraspanin (TM4SF) family. It is expressed in a variety of hematopoietic and epithelial cells. It displays strong expression in platelets where it mediates platelet activation and aggregation upon binding to monoclonal antibodies. CD9 induces cell-cell adhesion, platelet aggregation, and B cell development and is shown to associate with CD63, CD81, CD82, and CD36 and to bind to beta-1 integrins. CD9 activation of platelets is reported to be mediated by the Fc y RII (CD32) molecule. Downregulation of CD9 correlates with tumor progression in some forms of cancer. It can hamper metastasis formation by inhibiting integrin-mediated motility. Lower expression of CD9 are associated with weaker matrix adhesion and diffuse tumor cell growth in vitro.

Spécificité

Clone Z9.1 detects CD9 in its native, non-reduced form, in CD-9 positive cells amongst human PBMCs.

Immunogène

Intact LoVo colorectal carcinoma cells.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected CD9 in human tonsil, human breast cancer, and human skin tissues.

Flow Cytometry Analysis: A 1:1,000 dilution from a representative lot detected CD9 in human PBMCs.
human tonsil, human breast cancer, human liver cancer, human skin

This mouse monoclonal Anti-CD9 antibody, clone Z9.1, Cat. No. MABT914, is validated for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting for the detection of CD9.

Qualité

Evaluated by Immunohistochemistry in human human liver cancer tissue.

Immunohistochemistry Analysis: A 1:1000 dilution of this antibody detected CD9 in human liver cancer tissue.

Description de la cible

25.42 kDa calculated

Forme physique

Format: Purified

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1