Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

MABT887

Sigma-Aldrich

Anti-PL Scramblase 1 Antibody, clone 9A7

clone 9A7, from mouse

Synonyme(s) :

Phospholipid scramblase 1, PL scramblase 1, Ca(2+)-dependent phospholipid scramblase 1, Transplantability-associated protein 1, NOR1, TRA1

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

9A7, monoclonal

Espèces réactives

mouse

Technique(s)

western blot: suitable

Isotype

IgG1κ

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

mouse ... Plscr1(22038)

Description générale

Phospholipid scramblase 1 (UniProt Q9JJ00; also known as Ca(2+)-dependent phospholipid scramblase 1, Nor1, PL scramblase 1, Tra1, Transplantability-associated protein 1) is encoded by the Plscr1 (also known as Tra1b, Tras1) gene (Gene ID 22038) in murine species. Plasma membrane phospholipids are distributed asymmetrically between the inner and outer leaflets. Such asymmetrical distribution collapses in response to blood coagulation and apoptosis, resulting in phospholipid “scrambling” between the two leaflets of the plasma membrane. Flippases, floppases, and scramblases are three types enzymes known to mediate transbilayer lipid motion. Flippases and floppases function via ATP-dependent mechanism, while scramblases mediate transbilayer movement in a non-selective and energy-independent manner. Originally identified in 1996 as a 37 kDa erythrocyte type II transmembrane protein that mediates calcium-dependent membrane phospholipids redistribution, PL Scramblase 1 is the protein product encoded by the founding member of the PLSCR family of genes (PLSCR1-5). All PLSCR family members, with the exception of PLSCR2, possess a proline-rich N-terminal region containing PxxP and PPxY domains, a cysteine-rich region, a conserved calcium-binding domain (EF-hand-like), and a putative transmembrane region enriched in hydrophobic amino acids. In addition, PLSCR1 contains a nuclear localization signal (NLS) and a DNA-binding domain that are essential for its nuclear localization and associated nuclear function. In addition to PLSCRs, TMEM16 and XKR family members have also been reported to mediate scramblase activity.

Spécificité

Clone 9A7 reacts with murine PL Scramblase 1, and does not cross-reacts with murine PL Scramblase 2/3/4 or human PL Scramblase 1.

Immunogène

Epitope: Cytoplasmic domain
Recombinant protein corresponding to the cytoplasmic domain of mouse PL Scramblase 1.

Application

Anti-PL Scramblase 1 Antibody, clone 9A7 is an antibody against PL Scramblase 1 Antibody for use in Western Blotting.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)
Western Blotting Analysis: A representative lot detected PL Scramblase 1 in mouse lung homogenates. Clone 9A7 also detected LPS treatment-induced PL Scramblase 1 upregulation in liver using total tissue homogenates, as well as cytosolic and nuclear extracts from LPS-treated and untreated mice (Lu, B., et al. (2007). Biochim. Biophys. Acta. 1771(9):1177-1185).

Qualité

Evaluated by Western Blotting in mouse thymus tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected PL Scramblase 1 in 10 µg of mouse thymus tissue lysate.

Description de la cible

~38 kDa observed. Two additional bands shown correspond to heavy and light chains of endogenous IgG from the mouse thymus tissue.

Forme physique

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Vous ne trouvez pas le bon produit ?  

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique