MABS1145

Anti-3-PGDH Antibody, clone TK-08 13A8

clone TK-08 13A8, from mouse

• Synonyme(s) : D-3-phosphoglycerate dehydrogenase, 3-PGDH
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: TK-08 13A8, monoclonal
• Espèces réactives: human
• Technique(s): immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_006614
• Numéro d'accès UniProt: O43175
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PHGDH(26227)

Description

Description générale

D-3-phosphoglycerate dehydrogenase (EC1.1.1.95; UniProt O43175; also known as 3-PGDH, Epididymis secretory protein Li 113) is encoded by the PHGDH (also known as 3PGDH, HEL-S-113, NLS, NLS1, PGAD, PGDH, PDG, PGD, PGDH3, PHGDHD, SERA) gene (Gene ID 26227) in human. Originally described by Otto Warburg and now commonly known as the Warburg effect refers to the phenomenon that many tumor cells use aerobic glycolysis instead of mitochondrial respiration for energy production to support cell growth and proliferation. Enzymes involved in the complex network of metabolic pathways are therefore being studied as potential targets for cancer treatment. L-serine is an important precursor for macromolecule biosynthesis. 3-PGDH catalyzes the first and rate-limiting step of the serine synthesis pathway from 3-phosphoglycerate to phospho-hydroxypyruvate and is important during tumor development. The PHGDH gene is frequently amplified and the enzyme is overexpressed in 70% of the estrogen receptor-negative breast cancers, 39% of melanoma, and cervical cancers.

Immunogène

Recombinant protein corresponding to human 3-PGDH.

Application

Detect PHGDH using this mouse Monoclonal Anti-3-PGDH Antibody, clone TK-08 13A8, Cat. No. MABS1145, validated for use in Western Blotting.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected 3-PGDH in 10 µg HEK293 and Raji cell lysates.

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected 3-PGDH in human salivary gland tissue sections.

Qualité

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected 3-PGDH in 10 µg of HeLa cell lysate.

Description de la cible

~57 kDa observed

Forme physique

Format: Purified

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1