MABN722

Anti-WIF1 Antibody, clone 1G5

ascites fluid, clone 1G5, from mouse

• Synonyme(s) : WIF-1, WIF, Wnt inhibitory factor 1, WIF1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: ascites fluid
• Type de produit anticorps: primary antibodies
• Clone: 1G5, monoclonal
• Espèces réactives: mouse, human
• Technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG1
• Numéro d'accès UniProt: Q9Y5W5
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... WIF1(11197)

Description

Description générale

WIF1, also known as Wnt inhibitory factor 1 or WIF-1, is a secreted protein that binds to Wnt proteins and inhibits their activity. WNT proteins have been shown to act as an extracellular signaling molecules and may be involved in the control of embryonic development. WIf1 contains an N-terminal WIF domain and 5 epidermal growth factor (EGF)-like domains. It has been implied in recent sutyes that WIF1 expression is downregulated in many types of cancers.

Immunogène

Purified recombinant fragment of human WIF1 expressed in E. Coli.

Application

Anti-WIF1 Antibody, clone 1G5 is a highly specific mouse monoclonal antibody, that targets Wnt inhibitory factor 1 & has been tested in western blotting, IHC & Immunofluorescence.

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected WIF1 in HeLa cells.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected WIF1 in human ovarian tumor and lung cancer tissues.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Qualité

Evaluated by Western Blotting in HeLa, NIH/3T3 and NTERA-2 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected WIF1 in HeLa, NIH/3T3 and NTERA-2 cell lysates.

Description de la cible

~43 kDa observed. Uncharacterized bands may appear in some lysate(s).

Forme physique

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Remarque sur l'analyse

Control
HeLa, NIH/3T3 and NTERA-2 cell lysates

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable