MABN2330

Anti-Catenin delta-2 Antibody, clone 4H2.1

clone 4H2.1, from mouse

• Synonyme(s) : Delta-catenin, GT24, Neural plakophilin-related ARM-repeat protein, NPRAP, Neurojungin
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 4H2.1, monoclonal
• Espèces réactives: mouse, human
• Conditionnement: antibody small pack of 25 μg
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG2bκ
• Numéro d'accès NCBI: NP_001323
• Numéro d'accès UniProt: Q9UQB3
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CTNND2(1501)

Description

Description générale

Catenin delta-2 (UniProt: Q9UQB3; also known as Delta-catenin, GT24, Neural plakophilin-related ARM-repeat protein, NPRAP, Neurojungin) is encoded by the CTNND2 (also known as NPRAP) gene (Gene ID: 1501) in human. Delta Catenin is a component of the cadherin-catenin cell adhesion complex and is enriched in synaptosomes. Catenin Delta 2 is a member of the beta-catenin superfamily and is expressed in the brain where it is required for normal cognitive development and its highest expression is observed in fetal brain. Catenin Delta 2 is phosphorylated by Cdk5 and by GSK-3beta. Catenin Delta 2 plays a critical role in neuronal development, particularly in the formation and/or maintenance of dendritic spines and synapses. It is also involved in the regulation of Wnt signaling where it acts on beta-catenin turnover, facilitating beta-catenin interaction with GSK3beta, phosphorylation, ubiquitination, and degradation. When bound to ZBTB33 (Zinc Finger and BTB Domain Containing 33), it functions as a transcriptional activator. Catenin delta-2 also promotes the disruption of E-cadherin based adherens junction to favor cell spreading upon stimulation by hepatocyte growth factor. Two isoforms of Catenin delta-2 have been reported that are produced by alternative splicing. It has nine Armadillo (ARM) repeats and a coiled coil region (aa 49-84). Defects in CTNND2 gene are known to cause autism and delayed acquisition of psychomotor skills. (Ref.: Yuan, L et al. (2015). J. Biol. Chem. 290(17):10947-57).

Spécificité

Clone 4H2.1 specifically detects Catenin delta-2 in human and murine cells. It targets an epitope with in the C-tereminal region.

Immunogène

GST/His-tagged recombinant fragment corresponding to 145 amino acids from the C-terminal region of human Catenin delta-2.

Application

Anti-Catenin delta-2, clone 4H2.1 Antibody, Cat. No. MABN2330, is a highly specific mouse monoclonal antibody that targets Catenin delta-2 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution from a representative lot detected Catenin delta-2 in rat thalamus, rat cerebellum, and human cerebral cortex tissue sections.

Research Category
Neuroscience

Qualité

Evaluated by Western Blotting in mouse cerebellum tissue lysate.

Western Blotting Analysis: 0.02 µg/mL of this antibody detected Catenin delta-2 in mouse cerebellum tissue lysate.

Description de la cible

~150 kDa observed; 132.66 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1