MABN1584

Anti-Synapsin-2 Antibody, clone 19.21

clone 19.21, from mouse

• Synonyme(s) : Synapsin II, Synapsin-2
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 19.21, monoclonal
• Espèces réactives: rat, human, mouse
• Technique(s): activity assay: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_001029192
• Numéro d'accès UniProt: Q63537
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SYN2(6854)

Description

Description générale

Synapsin-2 (UniProt Q63537; also known as Synapsin II) is encoded by the Syn2 gene (Gene ID 29179) in rat species. The synapins constitute a family of abundant neuronal phosphoproteins that regulate SV trafficking and neurotransmitter release at the pre-synaptic terminal. Three genes exisit in mammals, encoding altogether 11 synapsin members by alternative splicings (Synapsin Ia & Ib by SYN1, Synapsin IIa & IIb by SYN2, Synapsin IIIa through IIIf by SYN3), Syn III is the most precociously expressed isoform that has a role in the early phases of neural development and is downregulated in mature neurons. On the other hand, Syn I and Syn II are expressed at low levels at birth and their expressions progressively increase during synaptogenesis to reach a stable plateau at 1–2 months of life. The NH2-terminal region is divided in A, B, and C domains and is highly conserved among synapsin isoforms. Synapsins are regulated by postransloational phosphorylations. Domain A contains PKA and CaMKI/IV phosphorylation sites, domain B contains MAPK/Erk phosphorylation sites, and domain C is phosphorylated by Src. The c-terminal regions contain spliced domains and diverge among synapsin isoforms (domain D in Syn Ia and Ib, domain G in Syn IIa and IIb, domain H in Syn IIa, and domain J in Syn IIIa), although they all bear proline-rich regions binding to several SH3-containing proteins and additional phosphorylation sites for CaMKII, MAPK/Erk, and cdk1/5.

Spécificité

Clone 19.21 reacts with both synapsin-2 spliced isoforms (IIa and IIb). Clone 19.21 does not react with any of the two synapsin-1 spliced isoforms (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).

Immunogène

Epitope: Near N-terminus

Purified recombinant rat synapsin-2.

Application

Anti-Synapsin-2 Antibody, clone 19.21 is an antibody against Synapsin-2 for use in Western Blotting, Immunohistochemistry (Paraffin), Immunoprecipitation, Enzyme Assay.

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected Synapsin-2 in human cerebral cortex, mouse cerebellum, and rat cerebral cortex tissue.
Western Blotting Analysis: A representative lot detected exogenously expressed wild-type and mutant (Y236S, G464R) human synapsin II in HeLa cells and Syn2-/- murine neurons transfected with the respective constructs (Corradi, A., et al. (2014). Hum. Mol. Genet. 23(1):90-103).
Western Blotting Analysis: A representative lot detected synapsin IIa/IIb, but not Ia/Ib, in rat brain cytosolic extracts (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
Immunoprecipitation Analysis: A representative lot immunoprecipitated synapsin IIa/IIb, but not Ia/Ib, from rat brain synaptosomal preparations (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).
Enzyme Assay Analysis: A representative lot, when added 2 hours prior to cAMP, prevented PKA-catalyzed phosphorylation of synapsin IIa/IIb in an in vitro kinase assay using rat brain synaptosomal preparations (Vaccaro, P., et al. (1997). Brain Res. Mol. Brain Res. 52(1):1-16).

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Qualité

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected Synapsin-2 in 10 µg of rat brain tissue lysate.

Description de la cible

~63 and 52 kDa observed

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable