MABF267
Anti-Cochlin Antibody/COCH, clone 9A10D2
clone 9A10D2, from rat
Synonyme(s) :
Cochlin, COCH-5B2
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rat
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
9A10D2, monoclonal
Espèces réactives
human, mouse
Technique(s)
immunohistochemistry: suitable
western blot: suitable
Isotype
IgG2aκ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... COCH(1690)
Description générale
The protein Cochlin/COCH is important in the development of the cochlea and vestibule in mammals as well as other functions. Cochlin/COCH is an extracellular matrix protein that plays a role in cell shape, positioning and motility. Recent research shows that it is also important in our innate immune response to bacteria and pathogens. Upon bacterial infection, a fragment of Cochlin/COCH is released from the conduits of B cell follicles in the spleen and this triggers protective cytokines in the periphery. Cochlin/COCH is expressed in epithelial tissues in various tissues, particularly in follicular cells and network of conduits in the spleen and lymph nodes. Cochlin/COCH also is a target for BMP4 mediation in embryonic stem cells (ESCs) where it facilitates self-renewal. The protein also appears to suppress neural differentiation of ESCs and suggests that Cochlin/COCH protein is important in ES cell fate determination. Mutations in the Cochlin/COCH gene are responsible for human DFNA9 syndrome, a disorder characterized by neurodegeneration of the inner ear that leads to hearing loss and vestibular impairments.
Immunogène
Recombinant protein corresponding to human Cochlin/COCH.
Application
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
This Anti-Cochlin Antibody/COCH, clone 9A10D2 is validated for use in Western Blotting and Immunohistochemistry for the detection of Cochlin/COCH.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Cochlin/COCH in 10 µg of human lymph node tissue lysate.
Western Blotting Analysis: A representative lot detected Cochlin/COCH in spleen and lymph node from WT and COCH knockout mice (Py, B.F., et al. (2013). Immunity. 38:1063-1072).
Western Blotting Analysis: A representative lot detected Cochlin/COCH in protein cleaved into p18 and p8 products. (Py, B.F., et al. (2013). Immunity. 38:1063-1072).
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Cochlin/COCH in mouse spleen tissue.
Immunohistochemistry Analysis: A representative lot detected Cochlin/COCH in spleen and lymphnode from WT and Coch knockout mice (Py, B.F., et al. (2013). Immunity. 38:1063-1072).
Western Blotting Analysis: A representative lot detected Cochlin/COCH in spleen and lymph node from WT and COCH knockout mice (Py, B.F., et al. (2013). Immunity. 38:1063-1072).
Western Blotting Analysis: A representative lot detected Cochlin/COCH in protein cleaved into p18 and p8 products. (Py, B.F., et al. (2013). Immunity. 38:1063-1072).
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Cochlin/COCH in mouse spleen tissue.
Immunohistochemistry Analysis: A representative lot detected Cochlin/COCH in spleen and lymphnode from WT and Coch knockout mice (Py, B.F., et al. (2013). Immunity. 38:1063-1072).
Qualité
Evaluated by Western Blotting in mouse spleen tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cochlin/COCH in 10 µg of mouse spleen tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cochlin/COCH in 10 µg of mouse spleen tissue lysate.
Description de la cible
~59 kDa observed. This protein is cleaved into p18 and p8 products. Uncharacterized bands may be observed in some lysates.
Forme physique
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Dorien Verdoodt et al.
International journal of molecular sciences, 22(21) (2021-11-14)
Several studies have shown that type IV fibrocytes, located in the spiral ligament, degenerate first after noise exposure. Interestingly, this is the region where Coch expression is most abundant. As it is suggested that cochlin plays a role in our
Charanjeet Kaur et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 43(50), 8801-8811 (2023-10-21)
Several lines of evidence have suggested that steeply sloping audiometric losses are caused by hair cell degeneration, while flat audiometric losses are caused by strial atrophy, but this concept has never been rigorously tested in human specimens. Here, we systematically
Qi Wang et al.
PloS one, 12(1), e0170011-e0170011 (2017-01-19)
By analyzing the different phenotypes of two Chinese DFNA9 families with the same mutation located in the intervening region between the LCCL and vWFA domains of cochlin and testing the functional changes in the mutant cochlin, we investigated the different
Richard Seist et al.
Frontiers in molecular neuroscience, 14, 670013-670013 (2021-06-11)
Cochlin is the most abundant protein in the inner ear. To study its function in response to noise trauma, we exposed adolescent wild-type (Coch +/+ ) and cochlin knock-out (Coch -/-) mice to noise (8-16 kHz, 103 dB SPL, 2
Rational design of a genomically humanized mouse model for dominantly inherited hearing loss, DFNA9.
Dorien Verdoodt et al.
Hearing research, 442, 108947-108947 (2024-01-14)
DFNA9 is a dominantly inherited form of adult-onset progressive hearing impairment caused by mutations in the COCH gene. COCH encodes cochlin, a crucial extracellular matrix protein. We established a genomically humanized mouse model for the Dutch/Belgian c.151C>T founder mutation in
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