MABF2299

Anti-IL-1α Antibody, clone 7E5.14.5

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 7E5.14.5, monoclonal
• Poids mol.: calculated mol wt 30.61 kDa; observed mol wt ~18 kDa
• Produit purifié par: using protein G
• Espèces réactives: human
• Conditionnement: antibody small pack of 100 μg
• Technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotype: IgG1κ
• Séquence de l'épitope: Unknown
• Numéro d'accès Protein ID: NP_000566.3
• Numéro d'accès UniProt: P01583
• Conditions d'expédition: 2-8°C
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

Interleukin-1 alpha (UniProt: P01583; also known as IL-1 alpha, Hematopoietin-1) is encoded by the IL1A (also known as IL1F1) gene (Gene ID: 3552) in human. IL-1a is constitutively expressed in several healthy tissues in a steady state and its expression can be increased in response to a number of stimuli. Absolute amounts of IL-1a vary among cell types, but barrier cells, such as endothelial and epithelial cells, express higher amounts at steady state. IL-1a is synthesized with a propeptide (aa 1-112) and is post-translationally modified by phosphorylation at serine 90, myristoylation on lysine 82, and acetylation on lysine 82. After synthesis, pro-IL-1a is localized into the nucleus, cytosol or lysosomal compartment or is displayed on the outer leaflet of the plasma membrane. Its intracellular localization can change in response to specific stimuli. Pro-IL-1a can also be cleaved by calpain to generate IL-1 -N-terminal domain (NTP) and a mature C-terminal IL-1a form. The C-terminal domain is catalytically active and is capable of signaling via IL-1R. Both IL-1a -NTP and pro-IL-1a contains a functional NLS signal and bind HAX1 to allow translocation into the nucleus. In the nucleus, IL-1a -NTP binds transcription regulation factors and activates expression of proinflammatory cytokines and chemokines independently of IL-1R1 signaling. The biological functions of IL-1a also involve stimulation of thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. (Ref.: Malik, A., and Kanneganti, TD. (2018). Immunol. Rev. 281(1); 124-137; Di Paolo, NC., and Shayakhmetov, DM (2016). Nat. Commun. 17(8); 906-913).

Spécificité

Clone 7E5.14.5 is a mouse monoclonal antibody that detects human IL-1a .

Immunogène

Full-length recombinant human IL-1alpha (without the propeptide).

Application

Quality Control Testing

Evaluated by Western Blotting with human recombinant IL-1a.

Western Blotting Analysis: A 1,000 dilution of this antibody detected human recombinant IL-1a.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-1a in human tonsil tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-IL-1a, clone 7E5.14.5, Cat. No. MABF2299, is a mouse monoclonal antibody that detects Interluekin-1a and is is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Forme physique

Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.

Stockage et stabilité

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable