MABC1145
Anti-FAP Antibody, clone 73.3
clone 73.3, from mouse
Synonyme(s) :
Fibroblast activation protein, Propyl endopeptidase FAP, Dipeptidyl peptidase FAP, Serine integral membrane protease, SIMP
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
73.3, monoclonal
Espèces réactives
mouse
Technique(s)
flow cytometry: suitable
Isotype
IgG1κ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
ambient
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
mouse ... Fap(14089)
Description générale
Fibroblast activation protein (UniProt: ; also known as Propyl endopeptidase FAP, Dipeptidyl peptidase FAP, Serine integral membrane protease, SIMP) is encoded by the Fap gene (Gene ID: 14089) in murine species. FAP is a homodimeric type II transmembrane protein that physically attaches to cells and bulk of the protein is exposed to extracellular space. It is a gelatinase of the serine protease family that is selectively expressed by tumor associated fibroblasts and pericytes in a majority of human epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. It is not expressed in most normal tissues, but is expressed during embryonic development. It is present as a homodimer and homodimerization is required for activity of both plasma membrane and soluble forms. It can also form heterodimer with DPP4. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences. Its gelatinase activity is inhibited by several serine-protease inhibitors, such as PMSF, AEBSF, and diisopropyl fluorophosphate (DFP). FAP is involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. It is also involved in the cell invasiveness towards the ECM in malignant melanoma cancers. (Ref.: Kelly, T et al. (2012). Int. Rev. Cell Mol. Biol. 297:83-116; Santos, AM et al. (2009). J. Clin. Invest. 119(12):3613-3625).
Spécificité
Clone 73.3 specifically detects fibroblast activation protein (FAP) in 3T3 cells overexpressing FAP.
Immunogène
FAP-expressing 3T3 cells.
Application
Detect fibroblast activation protein using this mouse monoclonal Anti-FAP Antibody, clone 73.3, Cat. No. MABC1145. It has been tested in Flow Cytometry.
Flow Cytometry Analysis: A representative lot detected FAP in Flow Cytometry applications (Wang, L.C., et. al. (2014). Cancer Immunol Res. 2(2):154-66).
Flow Cytometry Analysis: 1 µg/mL from a representative lot detected FAP in 3T3/FAP overexpressed cells, but not in 3T3 WT cells. (Courtesy of Leslie Hopper, University of Pennsylvania, USA).
Flow Cytometry Analysis: 1 µg/mL from a representative lot detected FAP in 3T3/FAP overexpressed cells, but not in 3T3 WT cells. (Courtesy of Leslie Hopper, University of Pennsylvania, USA).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Qualité
Isotype testing: Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .
Description de la cible
87.94 kDa calculated.
Forme physique
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Certificats d'analyse (COA)
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Cell reports, 36(3), 109422-109422 (2021-07-22)
Tumor-associated tertiary lymphoid structures (TA-TLS) are associated with enhanced patient survival and responsiveness to cancer therapies, but the mechanisms underlying their development are unknown. We show here that TA-TLS development in murine melanoma is orchestrated by cancer-associated fibroblasts (CAF) with
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