MAB1399Z-I

Anti-Integrin αX Antibody, clone N418 Antibody, azide free

clone N418, from hamster(Armenian)

• Synonyme(s) : Integrin alpha-X, CD11 antigen-like family member C, Leukocyte adhesion glycoprotein p150, 95 alpha chain, Leukocyte adhesion receptor p150, 95, CD11c
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: hamster (Armenian)
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: N418, monoclonal
• Espèces réactives: mouse
• Conditionnement: antibody small pack of 25 μg
• Technique(s): flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable
• Isotype: IgG
• Numéro d'accès NCBI: NP_067309
• Numéro d'accès UniProt: Q9QXH4
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Itgax(16411)

Description

Description générale

Integrin alpha-X (UniProt: Q9QXH4; also known as CD11 antigen-like family member C, Leukocyte adhesion glycoprotein p150,95 alpha chain, Leukocyte adhesion receptor p150,95, CD11c) is encoded by the Itgax gene (gene ID: 16411) in murine species. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. CD11c is a single-pass type I membrane protein. It has an extracellular domain (aa 20-1116), a transmembrane domain (aa 1117-1137), and a cytoplasmic domain (aa 1138-1169). It is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off in the mature form. Low levels of CD11c are expressed on human granulocytes and monocytes, and higher expression levels are observed on macrophages, dendritic cells and hairly cell leukemias. CD11c associates with beta-2 subunit in a non-covalent manner and then serves as a receptor for fibrinogen where it recognizes the sequence G-P-R. CD11c is shown to mediate cell-cell interaction during inflammatory responses and is important in monocyte adhesion and chemotaxis. Clone N418 is known to recognize an epitope on the p150 alpha chain. (Ref.: Metlay, JP et al. (1990). J. Exp. Med. 171(5); 1753-1771).

Spécificité

Clone N418 is a mouse monoclonal antibody that detects integrin alpha-X in murine tissue.

Immunogène

Mouse spleen dendritic cells.

Application

Anti-Integrin X, clone N418, azide free, Cat. No. MAB1399Z-I, is an armenian hamster monoclonal antibody that detects Integrin alpha-X and has been tested for use in Immunohistochemistry, Flow Cytometry, and Immunoprecipitation.

Immunoprecipitation Analysis: A representative lot detected Integrin X in Immunoprecipitation applications (Metlay, J.P., et. al. (1990). J Exp Med. 171(5):1753-71).

Immunohistochemistry Analysis: A representative lot detected Integrin X in Immunohistochemistry applications (Tan, C.S.E., et. al. (2017). PLoS Pathog. 13(4):e1006311; Roland, C.L., et. al. (2009). Mol Cancer Ther. 8(7):1761-71; Metlay, J.P., et. al. (1990). J Exp Med. 171(5):1753-71).

Flow Cytometry Analysis: A representative lot detected Integrin X in Flow Cytometry applications (Metlay, J.P., et. al. (1990). J Exp Med. 171(5):1753-71; Granucci, F., et. al. (1997). J Immunol. 159(4):1794-99; Cervantes-Barragan, L., et. al. (2007). Blood. 109(3):1131-37).

Research Category
Inflammation & Immunology

Qualité

Evaluated by Immunohistochemistry in mouse spleen frozen tissue sections.

Immunohistochemistry Analysis: A 1:25 dilution of this antibody detected Integrin X in mouse spleen frozen tissue sections.

Description de la cible

129.15 kDa calculated.

Forme physique

Format: Purified

Protein G purified

Purified armenian hamster monoclonal antibody IgG in PBS without preservatives.

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2