MAB1219
Anti-CD14 Antibody, clone 2D-15C
clone 2D-15C, Chemicon®, from mouse
Synonyme(s) :
LPS Receptor
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
2D-15C, monoclonal
Espèces réactives
human
Fabricant/nom de marque
Chemicon®
Technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
Isotype
IgG1
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... CD14(929)
Description générale
BIOCHEMISTRY: p I = 5.8
Spécificité
This antibody belongs to CD14 (assigned by the Third International Workshop on Leucocyte Differentiation Antigens, Oxford, 1986; McMichael et al. Eds., 1986). and reacts with 50 to 55kD protein. It will detect blood monocytes, Kupffer cells, red pulp macrophages, dendritic cells and also "epithelioid" or giant cells within granulomatous tissue (Hancock et al., 1983), foam cells in renal interstitium (Nolasco et al., 1984) and placental dendritic cells and macrophages (Barnett et al., 1984; Brooks et al., 1983; Polli et al., 1984; Hopper et al., 1986). Monocytes react with this antibody in the early stages of differentiation, promonocytes and monoblasts do not. Activity is not lost on monocyte/macrophage activation (Hancock et al., 1983).
Cell reactivity
Reacts with peripheral blood monocytes and weakly with granulocytes. Negative to platelets, erythrocytes and lymphocytes. Sometimes positive for chronic myeloid leukemia (CML) and acute myeloid leukemia (AML). Negative to B-cell chronic lymphocytic leukemia (B-CLL), T-cell chronic lymphocytic leukemia (T-CLL) and common acute lymphoblastic leukemia (CALL).
Cell reactivity
Reacts with peripheral blood monocytes and weakly with granulocytes. Negative to platelets, erythrocytes and lymphocytes. Sometimes positive for chronic myeloid leukemia (CML) and acute myeloid leukemia (AML). Negative to B-cell chronic lymphocytic leukemia (B-CLL), T-cell chronic lymphocytic leukemia (T-CLL) and common acute lymphoblastic leukemia (CALL).
Immunogène
Human peripheral blood mononuclear cells.
Application
Anti-CD14 Antibody, clone 2D-15C detects level of CD14 & has been published & validated for use in FC, IH.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
This antibody is an important general cell marker for mononuclear phagocytes in normal samples and a variety of disease states including a proportion of cases of myeloid leukemia. It is suitable for flow cytometry, and immunoperoxidase staining on frozen tissue sections.
SUGGESTED USAGE DILUTION
1. Flow cytometry and indirect immunofluorescence 1:25
Dilute with isotonic buffer. Use 50 μl per 1 x 106 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
2. Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:25 will be applicable to most commonly used systems.
SUGGESTED USAGE DILUTION
1. Flow cytometry and indirect immunofluorescence 1:25
Dilute with isotonic buffer. Use 50 μl per 1 x 106 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
2. Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:25 will be applicable to most commonly used systems.
Forme physique
Format: Purified
The purified antibody is supplied in phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.
Stockage et stabilité
Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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