HTS191RTA
Ready-to-Assay OXGR1 α-Ketoglutarate Receptor Frozen Cells
Human
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
41106514
eCl@ss :
32011203
Nomenclature NACRES :
NA.81
Produits recommandés
product name
Ready-to-Assay OXGR1 α-Ketoglutarate Receptor Frozen Cells,
Source biologique
human
Niveau de qualité
Fabricant/nom de marque
Ready-to-Assay
Technique(s)
calcium flux assay: suitable
Numéro d'accès NCBI
Méthode de détection
fluorometric
Description générale
Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
OXGR1 (also known as GPR99 or GPR80) was originally identified as an orphan with a sequence most closely related to P2Y receptors. Studies to deorphanize OXGR1 revealed that α-ketoglutarate activates OXGR1 through Gq to increase intracellular calcium (He et al., 2004; Qi et al., 2004; Abbracchio et al., 2005). The EC50 of OXGR1 for α-ketoglutarate is in the μM range, similar to the concentration of α-ketoglutarate in the circulation, indicating that the interaction is physiologically significant. Millipore′s cloned human OXGR1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant OXGR1-expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at OXGR1.
OXGR1 (also known as GPR99 or GPR80) was originally identified as an orphan with a sequence most closely related to P2Y receptors. Studies to deorphanize OXGR1 revealed that α-ketoglutarate activates OXGR1 through Gq to increase intracellular calcium (He et al., 2004; Qi et al., 2004; Abbracchio et al., 2005). The EC50 of OXGR1 for α-ketoglutarate is in the μM range, similar to the concentration of α-ketoglutarate in the circulation, indicating that the interaction is physiologically significant. Millipore′s cloned human OXGR1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant OXGR1-expression on the cell surface for functional detection via the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists, and modulators at OXGR1.
Description de la lignée cellulaire
- GPCR Cell Lines
- Host cells: Chem-1
Application
Human OXGR1, GPR99, GPR80 GPCR frozen cells for Calcium Flux FLIPR Assays.
Actions biochimiques/physiologiques
- GPCR Class: A
- Protein Target: OXGR, GPR99, GPR80
- Target Sub-Family: alpha-Ketoglutarate
Composants
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
Fifty (50) mL of Media Component.
Fifty (50) mL of Media Component.
Clause de non-responsabilité
This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique