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CC43

Sigma-Aldrich

Anti-Cdh1 (Ab-2) Mouse mAb (DH01)

liquid, clone DH01, Calbiochem®

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.43

Source biologique

mouse

Niveau de qualité

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

DH01, monoclonal

Forme

liquid

Contient

≤0.1% sodium azide as preservative

Espèces réactives

human

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

do not freeze

Isotype

IgG1

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... CDH1(999)

Description générale

Anti-Cdh1 (Ab-2), mouse monoclonal, clone DH01, recognizes the ~55 kDa Cdh-1 protein in Rat-1 cells. It is validated for Western blotting and immunoprecipitation.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the ~55 kDA Cdh1 protein.
Recognizes the ~55 kDa Cdh-1 protein in Rat-1 cells.

Immunogène

Human
recombinant human Cdh1 protein

Application


Immunoblotting (1 g/ml)
Immunoprecipitation (2 g/mg protein lysate)

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In 10 mM PBS, 0.2% BSA, pH 7.4.

Remarque sur l'analyse

Negative Control
Normal mouse serum
Positive Control
Junkat or LS174T cells

Autres remarques

Does not cross-react with other WD repeat containing proteins, including Cdc20. Antibody should be titrated for optimal results in individual systems.
Kramer, E.R., et al. 2000 Mol. Biol. Cell11, 1555.
Petersen, B.O., et al. 2000 Genes Dev.14, 2330.
Pfleger, C.M., et al. 2001 Genes Dev.15, 1759.
Sorensen, C.S., et al. 2001 Mol. Cell Biol.21, 3692.
Gieffers, C., et al. 1999 Proc. Natl. Acad. Sci. USA96, 11317.
Visintin, R., et al. 1997 Science278, 460.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Savvas C Pavlides et al.
Cell cycle (Georgetown, Tex.), 15(7), 931-947 (2016-03-11)
We previously reported that aberrant TGF-β/Smad2/3 signaling in endometrial cancer (ECA) leads to continuous ubiquitylation of p27(kip1)(p27) by the E3 ligase SCF-Skp2/Cks1 causing its degradation, as a putative mechanism involved in the pathogenesis of this cancer. In contrast, normal intact
W Liu et al.
Oncogene, 30(1), 21-31 (2010-08-31)
Inactivation of von Hippel-Lindau tumor-suppressor protein (pVHL) is associated with von Hippel-Lindau disease, an inherited cancer syndrome, as well as the majority of patients with sporadic clear cell renal cell carcinoma (RCC). Although the involvement of pVHL in oxygen sensing
Yu-Liang Kuo et al.
The EMBO journal, 25(8), 1741-1752 (2006-04-08)
The human T-lymphotropic virus type 1 (HTLV-1) Tax binds the anaphase promoting complex (APC) and activates it ahead of schedule. Here, we show that APC activation by Tax induces rapid senescence (tax-IRS) independently of p53 and pRB. In response to
Debjani Pal et al.
Cell death & disease, 11(4), 298-298 (2020-04-30)
APC/CCdh1 is a ubiquitin ligase with roles in numerous diverse processes, including control of cellular proliferation and multiple aspects of the DNA damage response. Precise regulation of APC/CCdh1 activity is central to efficient cell-cycle progression and cellular homeostasis. Here, we
Jie Xu et al.
Nature communications, 8, 14002-14002 (2017-01-17)
β-TrCP and SKP2 are two well-studied F-box proteins, which often act as oncogenes. Whether and how they communicate with each other is unknown. Here we report that FBXW2, a poorly characterized F-box, is a substrate of β-TrCP1 and an E3

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