ABS1623
Anti-HRD1 Antibody
from rabbit, purified by affinity chromatography
Synonyme(s) :
E3 ubiquitin-protein ligase synoviolin, Synovial apoptosis inhibitor 1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
mouse, human, rat
Réactivité de l'espèce (prédite par homologie)
rhesus macaque (based on 100% sequence homology)
Technique(s)
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... SYVN1(84447)
Description générale
E3 ubiquitin-protein ligase synoviolin (UniProt Q86TM6; also known as Synovial apoptosis inhibitor 1) is encoded by the SYVN1 (also known as HRD1, KIAA1810) gene (Gene ID 84447) in human. E3 ubiquitin-protein ligase synoviolin is a multi-transmembrane ER membrane protein. Originally named Hrd1, synoviolin adopted the current name due to its upregulation in synovial fibroblasts from patients with rheumatoid arthritis as a result of exposure to inflammatory cytokines. Synoviolin plays a protective role against ER stress-induced apoptosis by mediating not only the degradation of ER misfolded proteins, but also the degradation of proapoptotic protein p53 as well as IRE1α (inositol-requiring enzyme 1α), a critical kinase regulating ER stress-induced cell death. Synoviolin is produced with a signal peptide (a.a. 1-22) sequence, the removal of which yields the mature 5-transmembrane (a.a. 42-62, 99-119, 141-161, 170-190, 225-245) protein, having its N-terminal end in the ER lumen (a.a. 23-41) and C-terminal end (a.a. 246-617) in the cytoplasm. Its C-terminal cytoplasmic end contains the p53/TP53-interacting region (a.a. 236-270), an atypical RING-type zinc finger domain (a.a. 291-330), and a proline-rich region (a.a. 339-478).
Spécificité
Immunogen sequence is present in all three spliced isoforms of human HRD1/SYVN1 reported by UniProt (Q86TM6).
Immunogène
Linear peptide corresponding to the C-terminal sequence of human HRD1.
Application
Anti-HRD1 Antibody is an antibody against HRD1 for use in Western Blotting.
Western Blotting Analysis: A 1:200 dilution from a representative lot detected HRD1 in 10 µg of αT3-1 mouse pituitary gonadotroph cell lysate (Courtesy of Dr. Richard Wojcikiewicz, SUNY Upstate Medical University, Syracuse, NY).
Western Blotting Analysis: A representative lot detected HRD1 in the membrane, but not the cytosolic, fraction from RAT-1 rat fibroblasts (Pearce, M.M., et al. (2007). J. Biol. Chem. 282(28):20104-20115).
Western Blotting Analysis: A representative lot detected HRD1 in RAT-1 and HeLa whole cell lysates (Pearce, M.M., et al. (2007). J. Biol. Chem. 282(28):20104-20115).
Western Blotting Analysis: A representative lot detected HRD1 in SPFH2 immunoprecipitate from αT3-1 mouse pituitary gonadotroph cell lysate. The amount of co-immunoprecipitated HRD1 increased when using lysates from cells treated with proteasome inhibitor bortezomib (Cat. No. 504314) to increase cellular accumulation of ubiquitinated SPFH2 (Pearce, M.M., et al. (2007). J. Biol. Chem. 282(28):20104-20115).
Western Blotting Analysis: A representative lot detected HRD1 in the membrane, but not the cytosolic, fraction from RAT-1 rat fibroblasts (Pearce, M.M., et al. (2007). J. Biol. Chem. 282(28):20104-20115).
Western Blotting Analysis: A representative lot detected HRD1 in RAT-1 and HeLa whole cell lysates (Pearce, M.M., et al. (2007). J. Biol. Chem. 282(28):20104-20115).
Western Blotting Analysis: A representative lot detected HRD1 in SPFH2 immunoprecipitate from αT3-1 mouse pituitary gonadotroph cell lysate. The amount of co-immunoprecipitated HRD1 increased when using lysates from cells treated with proteasome inhibitor bortezomib (Cat. No. 504314) to increase cellular accumulation of ubiquitinated SPFH2 (Pearce, M.M., et al. (2007). J. Biol. Chem. 282(28):20104-20115).
Qualité
Evaluated by Western Blotting in αT3-1 mouse pituitary gonadotroph cell lysate.
Western Blotting Analysis: A 1:200 dilution of this antibody detected HRD1 in 10 µg of αT3-1 mouse pituitary gonadotroph cell lysate.
Western Blotting Analysis: A 1:200 dilution of this antibody detected HRD1 in 10 µg of αT3-1 mouse pituitary gonadotroph cell lysate.
Description de la cible
~74 kDa observed. 65.48 kDa (isoform 1), 59.53 kDa (isoform 2), and 65.41 kDa (isoform 3) calculated.
Forme physique
Purified rabbit polyclonal antibody in buffer containing 155 mM NaCl, 50 mM Tris-HCl pH 7.4 with 0.05% sodium azide and 10% glycerol.
Autres remarques
Concentration: Please refer to lot specific datasheet.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
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Qingguo Li et al.
Cell cycle (Georgetown, Tex.), 19(12), 1466-1477 (2020-04-21)
The 3-hydroxy-3-methylglutaryl reductase degradation (HRD1) is an E3 ubiquitin ligase that can preserve heart structure and function, but its role in endothelial dysfunction and atherosclerosis (AS) is unclear. The aim of this study was to explore the role and biological
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