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Key Documents

ABE48

Sigma-Aldrich

Anti-NELF-E Antibody

from rabbit, purified by affinity chromatography

Synonyme(s) :

Negative elongation factor E, NELF-E, RNA-binding protein RD

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

rat, human, mouse

Réactivité de l'espèce (prédite par homologie)

canine (based on 100% sequence homology), bovine (based on 100% sequence homology)

Technique(s)

immunocytochemistry: suitable
western blot: suitable

Numéro d'accès NCBI

NP_002895

Numéro d'accès UniProt

P18615

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... NELFE(7936)

Catégories apparentées

Description générale

NELF-E (Negative elongation factor E) is a main component of NELF human transcription factor complex which associates with DRB sensitivity-inducing factor to stifle transcriptional elongation by RNA polymerase II. NELF-E is an RNA-binding protein that includes arginine-aspartic acid dipeptide repeats. NELF-E RNA-binding activity is essential for NELF to function.

Immunogène

KLH-conjugated linear peptide corresponding to human NELF-E.

Application

Anti-NELF-E Antibody is a Rabbit Polyclonal Antibody for detection of NELF-E also known as Negative elongation factor E, NELF-E, RNA-binding protein RD & has been validated in ICC & WB.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected NELF-E in NIH/3T3 and HeLa cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Qualité

Evaluated by Western Blot in L6 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected NELF-E on 10 µg of L6 cell lysate.

Description de la cible

~ 43 kDa observed. Uncharacterized bands may be due to post-translational modification.

Forme physique

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
L6 cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes.
Stadelmayer, B; Micas, G; Gamot, A; Martin, P; Malirat, N; Koval, S; Raffel, R; Sobhian et al.
Nature Communications null
Seychelle M Vos et al.
eLife, 5 (2016-06-11)
Transcription regulation in metazoans often involves promoter-proximal pausing of RNA polymerase (Pol) II, which requires the 4-subunit negative elongation factor (NELF). Here we discern the functional architecture of human NELF through X-ray crystallography, protein crosslinking, biochemical assays, and RNA crosslinking

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