ABE219

Anti-phospho Histone H3 (Ser57) Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: mouse, human
• Technique(s): dot blot: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Numéro d'accès NCBI: NP_003520
• Numéro d'accès UniProt: P68431
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: phosphorylation (pSer57)
• Informations sur le gène: human ... HIST1H3F(8968)

Description

Description générale

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Immunogène

KLH-conjugated linear peptide corresponding to human Histone H3 phosphorylated at Ser57.

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho Histone H3 (Ser57) in HeLa cells.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated phospho Histone H3 (Ser57) from 500 µg of colcemid treated HeLa acid extract.

Dot Blot (Specificity) Analysis: 0.005 µg/mL from a representative lot detected phospho Histone H3 (Ser57) in a panel containing various modified and non-modified Histone H3 peptides.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

This Anti-phospho Histone H3 (Ser57) Antibody is validated for use in western blotting, ICC, IP & dot blot for the detection of phospho Histone H3 (Ser57).

Qualité

Evaluated by Western Blotting in colcemid treated HeLa acid extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected phospho Histone H3 (Ser57) in 10 µg of colcemid treated HeLa acid extract.

Description de la cible

~17 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Forme physique

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable