ABD124
Anti-Wnt3a Antibody
from rabbit, purified by affinity chromatography
Synonyme(s) :
Protein Wnt-3a
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.75
Source biologique
rabbit
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
human, mouse
Technique(s)
immunocytochemistry: suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... WNT3A(89780)
mouse ... Wnt3A(22416)
Description générale
Wnt-3a, also known as Protein Wnt3a, and encoded by the gene name Wnt3a and Wnt-3a, is a ligand for members of the frizzled family of seven transmembrane receptors and a member of the Wnt family. The Wnt family of consists of ligands involved in cell development, fate determination, organogenesis, and oncogenesis. Information on Wnt signaling has been inferred from genetic analysis of wingless (wg) signaling in Drosophila. The Wnt signaling cascade initiates at the cell membrane when Wnt interacts with its receptor, a member of the Frizzled (Fz) transmembrane receptor family and lipoprotein receptor–related proteins 5 and 6 (LRP-5/6). This interaction relays the signal into the interior of the cell where disheveled (dsh) is brought to the membrane and gets phosphorylated/activated. Dsh promotes the inactivation of GSK3 kinase activity, thus preventing the phosphorylation of β-Catenin and allowing it to accumulate as it avoids ubiquination and subsequent proteosome degradation. Stable β-Catenin accumulates in the cytoplasm where it interacts with lymphoid enhancer factor/T cell factor (LEF/TCF) and translocates into the nucleus. This results in activation of TCF-responsive genes which are thought to play key roles in development and cancer. Negative regulators of the pathway may include axin and the tumor suppressor APC. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Wnt-3a is expressed in dorsal portion of the neural tube (developing roof plate), and mesenchyme tissue surrounding the umbilical veins.
Immunogène
Linear peptide corresponding to mouse Wnt3a.
Application
Immunocytochemistry Analysis: A representative lot detected Wnt3a in COS7 cell lysate expressing WT Wnt3a (Kishida, S., et al. (2004). Mol Cell Biol. 10:4487-501).
Western Blotting Analysis: A representative lot detected Wnt3a in Cos-7 cell lysate overexpressing Wnt-3a (Sato, A., et al. (2010). EMBO J. 29(1):41-54).
Western Blotting Analysis: A representative lot detected Wnt3a in Cos-7 cell lysate overexpressing Wnt-3a (Sato, A., et al. (2010). EMBO J. 29(1):41-54).
Research Category
Stem Cell Research
Stem Cell Research
This Anti-Wnt3a antibody is validated for use in WB, IC for the detection of Wnt3a.
Qualité
Evaluated by Western Blotting in Cos 7 overexpressing mouse cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Wnt3a in 10 µg of Cos 7 overexpressing mouse cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Wnt3a in 10 µg of Cos 7 overexpressing mouse cell lysate.
Description de la cible
~39 kDa observed. Uncharacterized band(s) may appear in some lysates.
Forme physique
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Wei Feng et al.
Molecular medicine reports, 12(3), 4727-4733 (2015-06-23)
Previous studies have demonstrated that the Wnt/β‑catenin signaling pathway is critical to the induction and maintenance of chronic neuropathic pain caused by peripheral inflammation and nerve damage. Emerging evidence from recent studies suggests that epigenetic mechanisms may also be critical
C Hu et al.
Physiological research, 69(4), 701-710 (2020-06-26)
Chemotherapy-induced neuropathic pain (CNP) is the major dose-limiting factor in cancer chemotherapy. However, the mechanisms underlying CNP remain elusive. In the present study, CNP was induced by repeated intraperitoneal injection of vincristine (VCR) into male C57BL/6J mice. VCR administration caused
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