ABC227

Anti-GABARAPL2

from rabbit, purified by affinity chromatography

• Synonyme(s) : Gamma-aminobutyric acid receptor-associated protein-like 2, GABA(A) receptor-associated protein-like 2, Ganglioside expression factor 2, GEF-2, General protein transport factor p16, Golgi-associated ATPase enhancer of 16 kDa, GATE-16, MAP1 light chain 3-
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μL
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Numéro d'accès UniProt: P60520
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... GABARAPL2(11345)

Description

Description générale

Gamma-aminobutyric acid receptor-associated protein-like 2 (UniProt: P60520; also known as GABA(A) receptor-associated protein-like 2, Ganglioside expression factor 2, GEF-2, General protein transport factor p16, Golgi-associated ATPase enhancer of 16 kDa, GATE-16, MAP1 light chain 3-related protein) is encoded by the GABARAPL2 (also known as FLC3A, GEF2) gene (Gene ID: 11345) in human. GEF-2 is a momomeric ubiquitin-like modifier protein of ATG8 family that is involved in intra-Golgi traffic. It is shown to modulate intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF, which in turn stimulates the association with GOSR1. GEF-2 is also involved in autophagy and plays a role in mitophagy, which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level. It is ubiquitously expressed and higher expressions are observed in the brain, heart, prostate, ovary, spleen and skeletal muscle. Low expression levels are observed in lung, thymus, and small intestine. In the precursor molecule the peptide bond between aa 116 and 117 is cleaved off by ATG4 to generate mature protein.

Spécificité

This rabbit polyclonal antibody detects GABA(A) receptor-associated protein-like 2 (GABARAPL2) in human cells.

Immunogène

GST-tagged full length human recombinant GABA(A) receptor-associated protein-like 2 (GABARAPL2).

Application

Anti-GABARAPL2, Cat. No. ABC227, is a rabbit polyclonal antibody that detects Gamma-aminobutyric acid receptor-associated protein-like 2 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected GABARAPL2 in human cerebellum and human prostate tissue sections.

Qualité

Evaluated by Western Blotting in Raji membrane extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected GABARAPL2 in Raji membrane extract.

Description de la cible

~ 14 kDa observed; 13.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide/.

Autres remarques

Concentration: Please refer to lot specific datasheet.