ABC1392

Anti-PDGF-C (GFD)

from rabbit

• Synonyme(s) : Platelet-derived growth factor C, Fallotein, Spinal cord-derived growth factor, SCDGF, VEGF-E
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): monkey (based on 100% sequence homology)
• Conditionnement: antibody small pack of 25 μg
• Technique(s): western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NP_057289
• Numéro d'accès UniProt: Q9NRA1
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PDGFC(56034)

Description

Description générale

Platelet-derived growth factor C (UniProt: Q9NRA1; also known as PDGF-C, Fallotein, Spinal cord-derived growth factor, SCDGF, VEGF-E) is encoded by the PDGFC (also known as SCDGE, UNQ174/PRO200) gene (Gene ID: 56034) in human. PDGF-C is a disulfide-linked homodimeric growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. It also plays an important role in wound healing and angiogenesis. It also serves as a potent mitogen and chemoattractant for cells of mesenchymal origin. It is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off in the mature form. Its CUB domain is localized to amino acids 46 to 163 and proteolytic removal of the CUB domain is essential for unmasking the receptor-binding epitopes of the core domain. PDGF-C is expressed in the fallopian tube, vascular smooth muscle cells in kidney, breast and colon and in visceral smooth muscle of the gastrointestinal tract. Highly expressed in retinal pigment epithelia. Its high expression is also reported in retinal pigment epithelia. In the kidney, it is constitutively expressed in parietal epithelial cells of Bowman′s capsule, tubular epithelial cells, and in arterial endothelial cells. Higher levels of PDGF-C and have been reported in the malignant breast tumor microenvironment. In MCF-7 cells increased expression of PDGF-C is reported to enhance cell proliferation and tumor cell motility. Tissue plasminogen activator and matriptase are shown to process PDGF-C in MCF-7 cells to its active form. (Ref.: Hurst, NJ., et al. (2012). Biochem. J. 441(3); 909-918; Lei, H., and Kazlauskas, A (2008). Exp. Eye Res. 86(5); 711-712).

Spécificité

This rabbit polyclonal antibody detects full length platelet derived growth factor C (PDGF-C) and also the 18 kDa growth factor domain.

Immunogène

A linear peptide corresponding to 21 amino acids from the C-terminal growth factor domain of human Platelet-derived growth factor C (PDGF-C).

Epitope: C-terminus

Application

Anti-PDGF-C, Cat. No. ABC1392, is a rabbit polyclonal antibody that detects Platelet-derived growth factor C and has been tested for use in Western Blotting.

Research Category
Signaling

Western Blotting Analysis: A representative lot detected PDGF-C in Western Blotting applications (Hurst, N.J., et. al. (2012). Biochem J. 441(3):909-18).

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected PDGF-C in HEK293T cells. (Courtesy of Hyeong-Reh Choi Kim lab at Wayne State University).

Qualité

Evaluated by Western Blotting in HEK293T cells.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected PDGF-C in lysates from HEK293T cells.

Description de la cible

~48 kDa observed; 39.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable