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Merck
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Principaux documents

AB9662

Sigma-Aldrich

Anti-Tau phospho Serine 409 Antibody

Chemicon®, from rabbit

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

affinity purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

human

Fabricant/nom de marque

Chemicon®

Technique(s)

western blot: suitable

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

phosphorylation (pSer409)

Informations sur le gène

human ... MAPT(4137)

Description générale

Tau is a neuronal microtubule-associated protein found predominantly on axons and functions to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer′s disease (AD) brain. Hyperphosphorylated Tau is also found in neurofibrillary lesions in a range of other central nervous system disorders. Hyperphosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II (CK2), phosphorylate Tau. Serine 409 is phosphorylated by PKA in vitro and has shown to be phosphorylated in AD brain.

Spécificité

Tau phosphoSerine 409. The antibody recognizes Tau pSerine 409 in samples of recombinant human Tau treated with PKA for 45 minutes. The reactivity of the antibody is blocked with the pSerine 409 peptide but not the non-phosphopeptide or a generic phosphoSerine-containing peptide.

Immunogène

Synthetic peptide of amino acids surrounding the phosphoSerine 409 site of human Tau.

Application

Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
This Anti-Tau phospho Serine 409 Antibody is validated for use in WB for the detection of Tau phospho Serine 409.
Western blot: 1:1,000. Suggested blocking buffer is 5% BSA-TBST for one hour at room temperature. Suggested antibody dilution buffer is 1% BSA-TBST. Suggested antibody incubation time is 2 hours at room temperature.

Optimal working dilutions must be determined by the end user.

Stockage et stabilité

Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2


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Peripheral B cells may serve as a reservoir for persistent hepatitis C virus infection.
Ito, M; Masumi, A; Mochida, K; Kukihara, H; Moriishi, K; Matsuura, Y; Yamaguchi, K; Mizuochi, T
Journal of Innate Immunity null
Huey-Jiun Ko et al.
Journal of clinical medicine, 8(10) (2019-10-24)
Based on the protein kinase A (PKA)/GSK3β interaction protein (GSKIP)/glycogen synthase kinase 3β (GSK3β) axis, we hypothesized that these might play a role in Tau phosphorylation. Here, we report that the phosphorylation of Tau Ser409 in SHSY5Y cells was increased

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