AB5156
Anti-Calcium Channel Antibody, Voltage Gated α 1C
Chemicon®, from rabbit
Synonyme(s) :
L-type
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
rat, rabbit, mouse, human
Fabricant/nom de marque
Chemicon®
Technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... CACNA1C(775)
Description générale
Voltage-sensitive calcium channels (vscc) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1c gives rise to l-type calcium currents. Long-lasting (l-type) calcium channels belong to the "high-voltage activated" (hva) group. They are blocked by dihydropyridines (dhp), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-aga-IIIA). They are however insensitive to omega-conotoxin- gvia (omega-ctx-gvia) and omega-agatoxin-IVA (omega-aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to dhp compounds (Swiss Prot).
Spécificité
Recognizes alpha 1C subunit (both LMW and HMW forms) of voltage-gated calcium channel. Does not cross react with any other calcium channel antigens tested so far. Guinea pig (17/18 residues identical); human and rabbit (16/18 residues identical).
Immunogène
Purified peptide (C)TTKI NMDDL QPSEN EDKS (CNC1) from 848-865 of alpha 1C subunit of rat brain voltage-gated calcium channel (Accession number P22002). Epitope location is in the intracellular loop between domains II and III.
Application
Alpha 1 subunits of voltage-gated Ca2+ channels are highly sensitive to proteases. All procedures that are going to receive a full-length protein should be performed at 4°C, and the following protease inhibitor mixture should be used: pepstatin A (1 μg/mL), leupeptin (1 μg/mL), aprotinin (1 μg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 μg/mL each).
Immunohistochemistry: 1:200 on rat brain sections.
Western blot: 1:200 using ECL on rat brain and heart material. LMW form shows a 190kDa band while the HMW form shows a 210kDa band.
Immunoprecipitation
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions and protocols must be determined by the end user.
Immunohistochemistry: 1:200 on rat brain sections.
Western blot: 1:200 using ECL on rat brain and heart material. LMW form shows a 190kDa band while the HMW form shows a 210kDa band.
Immunoprecipitation
Dilutions should be made using a carrier protein such as BSA (1-3%).
Optimal working dilutions and protocols must be determined by the end user.
Detect Calcium Channel using this Anti-Calcium Channel Antibody, Voltage Gated α 1C, validated for use in IH, IP & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Signaling Neuroscience
Ion Channels & Transporters
Signaling Neuroscience
Description de la cible
190 & 210 kDa
Forme physique
Affinity Purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
ImmunoAffinity Purified
Stockage et stabilité
Maintain lyophilized material at -20°C for up to 12 months. After reconstitution add glycerol (ASC grade or better) at a ratio of 1:1 and maintain at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Remarque sur l'analyse
Control
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
CONTROL ANTIGEN: Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 3
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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State-dependent signaling by Cav1.2 regulates hair follicle stem cell function.
Yucel, G; Altindag, B; Gomez-Ospina, N; Rana, A; Panagiotakos, G; Lara, MF; Dolmetsch, R; Oro, AE
Genes & Development null
Wei Wang et al.
Molecular medicine reports, 14(4), 3848-3854 (2016-09-08)
Remodeling of atrial electrophysiology and structure is the primary feature of atrial fibrillation (AF). Evidence suggests that abnormalities in the expression levels of embryological cardiovascular development‑associated transcription factors, including Nkx2.5, are crucial for the development of AF. Rat atrial myocardial
R A Morton et al.
Neuroscience, 238, 59-70 (2013-02-19)
Voltage-gated calcium channels (VGCCs) play a major role during the development of the central nervous system (CNS). Ca(2+) influx via VGCCs regulates axonal growth and neuronal migration as well as synaptic plasticity. Specifically, L-type VGCCs have been well characterized to
Georgia Panagiotakos et al.
eLife, 8 (2019-12-24)
The syndromic autism spectrum disorder (ASD) Timothy syndrome (TS) is caused by a point mutation in the alternatively spliced exon 8A of the calcium channel Cav1.2. Using mouse brain and human induced pluripotent stem cells (iPSCs), we provide evidence that
Huan Chen et al.
Nature communications, 11(1), 4328-4328 (2020-08-30)
The general anesthetic ketamine has been repurposed by physicians as an anti-depressant and by the public as a recreational drug. However, ketamine use can cause extensive pathological changes, including ketamine cystitis. The mechanisms of ketamine's anti-depressant and adverse effects remain
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