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AB2999

Sigma-Aldrich

Anti-BNIP3 Antibody

from rabbit, purified by affinity chromatography

Synonyme(s) :

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Clone:
polyclonal
application:
ICC
WB
Espèces réactives:
human
Technique(s):
immunocytochemistry: suitable
western blot: suitable
citations:
2

Source biologique

rabbit

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

human

Réactivité de l'espèce (prédite par homologie)

mouse (based on 100% sequence homology)

Technique(s)

immunocytochemistry: suitable
western blot: suitable

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... BNIP3(664)

Description générale

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (BNIP3) is a member of the NIP3 family, a singular group of death-inducing, mitochondrial proteins. BNIP3 is comprised of several domains of interest; a highly conserved domain (CD), a C-terminal transmembrane (TM) domain, and a BH3 domain. Strongly upregulated by hypoxia, BNIP3 induces a caspase-independent cell death characterized by mitochondrial damage. Overexpression has been observed in the brain after stroke and in the adult heart following myocardial infarction. Recent studies have shown BNIP3 to be involved in cell growth inhibition through suppression of the mTOR pathway and that, when localized to the nucleus, it has the paradoxical capability of repressing apoptosis in tumors by binding the AIF expression gene.

Spécificité

Other homologies: Rat, equine, and ox (90% sequence homology).

Immunogène

Epitope: Internal (C-terminal half).
KLH-conjugated linear peptide corresponding to an internal sequence within the C-terminal half of human BNIP3.

Application

Anti-BNIP3 Antibody detects level of BNIP3 & has been validated for use in Immunocytochemistry and Western Blotting.
Immunocytochemistry Analysis: A representative lot detected hypoxia-induced BNIP3 upregulation in human glioblastoma-astrocytoma, epithelial-like U87 cells (Courtesy of Spencer Gibson, Manitoba Institute of Cell Biology).
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Qualité

Evaluated by Western Blotting in PC3 cell lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected BNIP3 in 10 µg of PC3 cell lysate.

Description de la cible

~60 kDa (dimer) observed. BNIP3 may be observed as a 30 kDa monomer and/or a 60 kDa dimer.

Forme physique

Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
PC3 cell lysate.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Y Yuan et al.
Cell death & disease, 4, e690-e690 (2013-06-29)
The histone methyltransferase G9a is overexpressed in a variety of cancer types, including pancreatic adenocarcinoma, and promotes tumor invasiveness and metastasis. We recently reported the discovery of BRD4770, a small-molecule inhibitor of G9a that induces senescence in PANC-1 cells. We
Lisa A Brennan et al.
Experimental eye research, 174, 173-184 (2018-06-08)
The formation and life-long growth of the ocular lens depends on the continuous differentiation of lens epithelial cells into lens fiber cells. To achieve their mature structure and transparent function, newly formed lens fiber cells undergo a series of cellular

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