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Sigma-Aldrich

BL21 Competent Cells - Novagen

Escherichia coli, rod shaped

Synonyme(s) :

BL21 cell line, Novagen competent cells

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About This Item

Code UNSPSC :
41106202
Nomenclature NACRES :
NA.81

product name

BL21 Competent Cells - Novagen, BL21 host strain is the most widely used host background and has the advantage of being deficient in both lon and ompT proteases.

Source biologique

Escherichia coli

Niveau de qualité

100

Fabricant/nom de marque

Novagen®

Conditions de stockage

OK to freeze
avoid repeated freeze/thaw cycles

Mode de croissance

adherent or suspension

Morphologie

rod shaped

Technique(s)

microbiological culture: suitable

Transformation cellulaire

transformation efficiency: >2×107 cfu/μg

Conditions d'expédition

dry ice

Température de stockage

−70°C

Description générale

BL21 host strain is the most widely used host background and has the advantage of being deficient in both lon and ompT proteases.
BL21 is the most widely used host background and has the advantage of being deficient in both lon (1) and ompT proteases.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Composants

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlBL21 Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•10 µl10 µlTest Plasmid

Avertissement

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Autres remarques

1. Phillips, T. A., Van Bogelen, R. A., and Neidhardt, F. C. (1984) J. Bacteriol.
159, 283–287.

Informations légales

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Pinku Sarmah et al.
Cell reports, 42(3), 112140-112140 (2023-02-27)
Signal-sequence-dependent protein targeting is essential for the spatiotemporal organization of eukaryotic and prokaryotic cells and is facilitated by dedicated protein targeting factors such as the signal recognition particle (SRP). However, targeting signals are not exclusively contained within proteins but can
Michio Nakaya et al.
Methods in molecular biology (Clifton, N.J.), 746, 317-327 (2011-05-25)
Heterotrimeric G proteins are composed of α, β, and γ subunits. G proteins can be activated by a large number of cell-surface hepathelical receptors and can transduce signals from these receptors to various intracellular signaling molecules. When G protein-coupled receptors
John Maciejowski et al.
Developmental cell, 41(2), 143-156 (2017-04-26)
The spindle assembly checkpoint kinase Mps1 not only inhibits anaphase but also corrects erroneous attachments that could lead to missegregation and aneuploidy. However, Mps1's error correction-relevant substrates are unknown. Using a chemically tuned kinetochore-targeting assay, we show that Mps1 destabilizes microtubule attachments

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