676472

VEGF₁₆₅, Human, Recombinant, S. frugiperda

• Synonyme(s) : Vascular Endothelial Growth Factor₁₆₅, rhVEGF, rhVPF, rh-Vasculotropin
• Code UNSPSC : 12352202

Propriétés

• Essai: ≥97% (SDS-PAGE)
• Niveau de qualité: 100
• Forme: lyophilized
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Impuretés: ≤1 EU/μg Endotoxin (EU/μg VEGF)
• Conditions d'expédition: ambient
• Température de stockage: −70°C

Description

Description générale

Recombinant, human VEGF₁₆₅ (165 amino acid splice variant of VEGF) expressed in S. frugiperda insect cells. A heparin-binding glycoprotein with potent angiogenic, endothelial cell-specific mitogenic, and vascular permeability-enhancing activities. Increases endothelial cell proliferation after vascular injury. Supplied as a glycosylated, disulfide-linked homodimer.

Actions biochimiques/physiologiques

ED₅₀ = 2.0-6.0 ng/ml as measured by its ability to stimulate ³H-thymidine incorporation into human umbilical vein endothelial cells

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Lyophilized from sterile-filtered 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg VEGF.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -70°C.

Reconstitute to a final concentration of ≥1 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.

Autres remarques

Liu, X., et al. 2001. J. Urology166, 354.
Burke, P.A., et al. 1995. Biochem. Biophys. Res. Commun. 207, 348.
Neufeld, G., et al. 1994. Prog. Growth Factor Res.5, 89.
Leung, D.W., et al. 1989. Science 246, 1306.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3