516560
Pertussis toxin
from Bordetella pertussis, Five distinct bands by SDS-PAGE, lyophilized, protein endotoxin, Calbiochem
Synonyme(s) :
Pertussis Toxin, Bordetella pertussis
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About This Item
Produits recommandés
product name
Pertussis Toxin, Bordetella pertussis, This Pertussis toxin, CAS 70323-44-3, catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins Gi, Go, and Gt.
Niveau de qualité
Forme
lyophilized
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
do not freeze
Activité étrangère
adenylate cyclase ≤35 pmol/min-μg (in the presence of calmodulin)
Conditions d'expédition
ambient
Température de stockage
2-8°C
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Description générale
Pertussis toxin (PT), secreted protein exotoxin and virulence factor by Bordetella pertussis, is the causative agent of whooping cough. It is transported across the bacterial outer membrane through a type IV secretion system encoded by the ptl genes, situated downstream from the ptx genes that encode the toxin. This complex multisubunit toxin with an AB5 configuration, serves as a crucial component of acellular pertussis vaccines. It can function protectively as a single-component toxoid vaccine. Moreover, it is widely used in mammalian cell signaling studies due to its inhibitory effect on G-protein-coupled receptor pathways. It inhibits neutrophil recruitment and airway influx, a common inflammatory response to bacterial infection.
Application
Pertussis Toxin, Bordetella pertussis has been used as a Gi protein inhibitor to study its ability to reduce the depolarizing responses of hypothalamic pro-opiomelanocortin (POMC) neurons. It has also been used in pertussis toxin experiments to block Gαi for an in vivo inner ear and an in vitro outer hair cell (HC) study.
Actions biochimiques/physiologiques
Primary Target
Guanine nucleotide-binding regulatory proteins Gi, Go, and Gt
Guanine nucleotide-binding regulatory proteins Gi, Go, and Gt
Product does not compete with ATP.
Avertissement
Toxicity: Toxic (F)
Forme physique
Lyophilized from 50 mM NaCl, 10 mM sodium phosphate buffer, pH 7.0.
Reconstitution
Following reconstitution, refrigerate (4°C). For long-term storage in solution, reconstitute with a high ionic strength buffer, such as 100 mM sodium phosphate, 500 mM sodium chloride, pH 7.0. Stock solutions are stable for at least 6 months for use in CHO cell assays and for 1-2 months for use in non-cellular systems.
Reconstitute with 500 µl H₂O. Obtain a uniform suspension before each use by scraping the walls of the tube and gently pipetting the solution to mix.
Remarque sur l'analyse
Five distinct bands by SDS-PAGE
Autres remarques
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Joshi, S.A., et al. 1998. FEBS Lett. 441, 67.
Fryer, M.W. 1992. Neurosci. Lett.146, 84.
Gierschik, P. 1992. Curr. Topics Microbiol. Immunol.175, 69.
Harada, J., et al. 1992. Eur. J. Pharmacol.227, 301.
Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem.267, 13735.
Kaslow, H.R., and Burns, D.L., 1992. FASEB J.6, 2684.
Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap.263, 1479.
Hewlett, E.L., et al. 1983. Infect. Immun.40, 1198.
Moss, J., et al. 1983. J. Biol. Chem.258, 11879.
Fryer, M.W. 1992. Neurosci. Lett.146, 84.
Gierschik, P. 1992. Curr. Topics Microbiol. Immunol.175, 69.
Harada, J., et al. 1992. Eur. J. Pharmacol.227, 301.
Hausman, S.Z., and Burns, D.L. 1992. J. Biol. Chem.267, 13735.
Kaslow, H.R., and Burns, D.L., 1992. FASEB J.6, 2684.
Zhu, J., et al. 1992. J. Pharmacol. Exp. Therap.263, 1479.
Hewlett, E.L., et al. 1983. Infect. Immun.40, 1198.
Moss, J., et al. 1983. J. Biol. Chem.258, 11879.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Code de la classe de stockage
13 - Non Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Pertussis Toxin: Structure-Function Relationship
Locht C, et al.
Bacterial Protein Toxins, 167-185 (2000)
Live imaging of hair bundle polarity acquisition demonstrates a critical timeline for transcription factor Emx2
Tona Y and Wu DK
eLife, 9, e59282-e59282 (2020)
Nicholas H Carbonetti
Future microbiology, 5(3), 455-469 (2010-03-10)
Pertussis toxin and adenylate cyclase toxin are two important virulence factors of Bordetella pertussis, the bacterial cause of the respiratory disease pertussis or whooping cough. In addition to studies on the structure, function and role in pathogenesis of these two
P Órdenes et al.
Scientific reports, 11(1), 21644-21644 (2021-11-06)
Previous studies indicate that the activity of hypothalamic POMC neurons can be regulated by glucose via intracellular mechanisms, but its regulation by lactate is poorly understood. In addition to its energetic role, lactate acts as a signaling molecule. In this
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