480025

NEMO-Binding Domain Binding Peptide, Cell-Permeable

A cell-permeable Antennapedia-NBD (wild type) fusion peptide that exhibits anti-inflammatory properties.

• Synonyme(s) : NEMO-Binding Domain Binding Peptide, Cell-Permeable, DRQIKIWFQNRRMKWKKTALDWSWLQTE, NBD-Binding Peptide, Cell Permeable
• Formule empirique (notation de Hill): C₁₇₀H₂₅₉N₄₉O₄₂S
• Poids moléculaire : 3693.24
• Code UNSPSC : 41116107

Propriétés

• Niveau de qualité: 100
• Essai: ≥95% (HPLC)
• Forme: lyophilized solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated (hygroscopic)
• Couleur: white
• Solubilité: DMSO: 50 mg/mL
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

A cell-permeable Antennapedia-NBD (NEMO binding domain) (wild type) fusion peptide that exhibits anti-inflammatory activity in mouse model of acute inflammation. NBD is an amino terminal α-helical region of the NEMO (NF-κB essential modifier; IKKγ) associated with a carboxyl-terminal segment of IKKα and IKKβ. This peptide blocks the association of NEMO with the IKK complex and prevents NF-κB activation. NEMO is also reported to suppress the intrinsic basal activity of the IKK complex. Its anti-inflammatory properties are attributed to the inhibition of cytokine-induced NF-κB activation and NF-κB-dependent gene expression. Does neither affect the TNF-α-induced phosphorylation of c-Jun nor the DNA binding of Oct-1 transcription factor.

A cell-permeable Antennapedia-NBD (wild type) fusion peptide that exhibits anti-inflammatory properties. Acts by inhibiting cytokine-induced NF-κB activation and NF-κB-dependent gene expression. Suggested to block the interaction of NEMO with the IKK (IκB)-kinase complex and thereby prevents NF-κB activation. Does not affect the TNF-α-induced phosphorylation of c-Jun and DNA binding of the transcription factor Oct-1.

Actions biochimiques/physiologiques

Cell permeable: yes

Primary Target
cytokine-induced NF-κB activation and NF-κB-dependent gene expression

Product does not compete with ATP.

Reversible: no

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Standard Handling (A)

Séquence

H-Asp-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-Thr-Ala-Leu-Asp-Trp-Ser-Trp-Leu-Gln-Thr-Glu-OH

Forme physique

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Autres remarques

Jimi, E., et al. 2004. Nat. Med., 10, 617.
Siegmund, D., et al. 2001. J. Biol. Chem.276, 43708.
May, M.J., et al. 2000. Science289, 1550.
Li, Q., et al. 1999. Science284, 321.

Note: This peptide will precipitate when added to tissue culture medium even at 100 µM concentrations. However, sufficient material will stay in the medium to permeate cells.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable