407673

Interleukin-8, Human, Recombinant, E. coli

• Synonyme(s) : rhIL-8
• Code UNSPSC : 12352202

Propriétés

• Pureté: ≥97% (SDS-PAGE)
• Niveau de qualité: 100
• Forme: lyophilized
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Impuretés: ≤100 EU/μg Endotoxin (EU/μg IL-8)
• Conditions d'expédition: ambient
• Température de stockage: −70°C

Description

Description générale

Recombinant, human interleukin-8 expressed in E. coli. A potent proinflammatory cytokine produced by monocytes, fibroblasts, and keratinocytes in response to stimulation by LPS, IL-1, or TNF-α, and by T lymphocytes in response to PHA stimulation. Induces adhesion of neutrophils to endothelial cells. A physiological initiator of chemotactic migration of leukocytes. May be involved in chronic inflammation. IL-8 acts as an angiogenic factor in human gastric carcinoma.

Recombinant, human interleukin-8 expressed in E. coli. IL-8 is produced by monocytes, fibroblasts, and keratinocytes in response to stimulation by LPS, IL-1, or TNFα, and by T lymphocytes in response to PHA stimulation. Induces adhesion of neutrophils to endothelial cells. May be involved in chronic inflammation.

Actions biochimiques/physiologiques

ED₅₀ = 150-300 ng/ml as measured by induction of myeloperoxidase release from human neutrophils

Avertissement

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.

Reconstitute to a concentration ≥10 µg/ml with sterile PBS containing at least 0.1% HSA or BSA.

Autres remarques

Kido, S., et al. 2001. Eur. J. Cancer37, 1482.
Wilson, A.J., et al. 1999. Clin. Sci. 97, 385.
Harada, A., et al. 1994. J. Leukoc. Biol.56, 559.
Mazzucchelli, L., et al. 1994. Am. J. Pathol. 144, 997.
Oppenheim, J.J., et al. 1991. Annu. Rev. Immunol.9, 617.
Matsushima, K., et al. 1988. J. Exp. Med.167, 1883.
Schröeder, J., et al. 1987. J. Immunol.139, 3474.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable