407252

Insulin-Like Growth Factor-I, Mouse, Recombinant, E. coli

• Synonyme(s) : mIGF-I
• Code UNSPSC : 12352202

Propriétés

• Essai: ≥98% (SDS-PAGE and HPLC)
• Niveau de qualité: 100
• Forme: lyophilized
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Impuretés: ≤1 ng/μg Endotoxin (ng/μg IGF-I)
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

Full-length, recombinant, mouse insulin-like growth factor I (IGF-I) expressed in E. coli. IGF-I regulates proliferation and survival signals through the Akt pathway. Mouse IGF-I shares 94% sequence identity with human IGF-I and exhibits cross-species reactivity. Useful in the study of cell proliferation and survival signals.

Actions biochimiques/physiologiques

ED₅₀: ≤2 ng/ml (corresponding to a specific activity of ≥1 x 10⁶ units/mg) as determined by a cell proliferation assay using FDC-P1 cells. For most in vitro applications IGF-I exerts is biological activity in the concentration range of 1-20 ng/ml.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Sterile-filtered prior to lyophilization. Contains no additives.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions. Aliquots may be stored at 4°C for up to 1 week without loss of biological activity.

Reconstitute with sterile H₂O to a final concentration of 0.1-1 mg/ml. Further dilute with aqueous buffer before use.

Autres remarques

Clemmons, D.R., et al. 2006. Curr. Opin. in Pharmacol. 6, 620.
Bell, G.I., et al. 1986. Nucleic Acids Res.14, 7873.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable