324904

Epiregulin, Mouse, Recombinant

Epiregulin, Mouse, Recombinant is expressed in E. coli with an N-terminal methionine. Inhibits epithelial tumor growth and stimulates the growth of fibroblasts and other cell types.

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Produit recombinant: expressed in E. coli
• Niveau de qualité: 100
• Essai: ≥97% (SDS-PAGE)
• Forme: liquid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Impuretés: <1.0 EU/μg protein Endotoxin
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

Recombinant, mouse epiregulin expressed in E. coli with an N-terminal methionine. Epiregulin is shown to inhibit epithelial tumor growth, able to stimulate growth of fibroblasts and other cell types, and has been shown to be an autocrine growth factor in human epidermal keratinocytes.

Actions biochimiques/physiologiques

ED₅₀ = 0.3-1 ng/ml as measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Lyophilized from 0.2 µm sterile-filtered PBS, BSA.

Reconstitution

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 10 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

Autres remarques

Shirakata, Y., et al. 2000. J. Biol. Chem.275, 5748.
Shelly, M., et al. 1998. J. Biol. Chem.273, 10496.
Das, S.K., et al. 1997. Dev. Biol.190, 178.
Komurasaki, T., et al. 1997. Oncogene15, 2841.
Toyoda, H., et al. 1995. J. Biol. Chem.270, 7495.
Toyoda, H., et al. 1995. FEBS Lett.377, 403.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable