2102-M
Protein-Concentrate Kit (Med)
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About This Item
Code UNSPSC :
12161503
eCl@ss :
32161000
Nomenclature NACRES :
NA.27
Produits recommandés
Application(s)
sample preparation
Description générale
The Chemicon Protein-Concentrate kit uses a proprietary reagent Universal Protein Precipitation Agent (UPPA). Protein solutions as dilute as 1 ng/mL can be quantitatively concentrated into a small volume. Protein precipitation is not effected by the presence of detergents, chaotropic, or other common laboratory agents. After precipitation, the precipitate is suspended in a small volume of Precipitate Suspension Buffer supplied with each kit. If the protocol is followed correctly, the recovery is generally 100%. There are enough reagents for concentrating up to 30 mL of dilute protein solution.
Application
Suitable for concentrating proteins for running gels, raising antibodies, protein purification, protein assays and other applications.
This kit is not suitable for those proteins which may lose some of their biological activities when precipitated.
PROTOCOL: ImportantPerform the entire procedure in the cold (ice bucket) unless specified otherwise. Concentration should be performed in a centrifuge tube. For small volumes, use microfuge tubes. Always position microfuge tubes in the centrifuge at the same orientation, i.e. cap-hinge facing out-ward, this will allow the pellet to remain glued to the same side of the tube during repeated centrifugations and minimize the loss of protein pellets. See product insert for protocol.
This kit is not suitable for those proteins which may lose some of their biological activities when precipitated.
PROTOCOL: ImportantPerform the entire procedure in the cold (ice bucket) unless specified otherwise. Concentration should be performed in a centrifuge tube. For small volumes, use microfuge tubes. Always position microfuge tubes in the centrifuge at the same orientation, i.e. cap-hinge facing out-ward, this will allow the pellet to remain glued to the same side of the tube during repeated centrifugations and minimize the loss of protein pellets. See product insert for protocol.
Conditionnement
1 kit
Composants
UPPA-I 100 mL UPPA-II 100 mL OrgoSol 2 x 50 mL UPC-SEED 2 x 0.30 mL Precipitate Buffer I 2 x 2.0 mL Precipitate Buffer II 2 x 0.5 mL UCP™-Wash 2 x 2.0 mL
Stockage et stabilité
Store at room temperature for up to 6 months from date of purchase. To avoid possible microbial contamination, dispense all solutions aseptically.
Informations légales
UCP is a trademark of Intelligent Material Solutions, Inc.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3
Organes cibles
Central nervous system, Respiratory system
Code de la classe de stockage
3 - Flammable liquids
Point d'éclair (°F)
-2.2 °F
Point d'éclair (°C)
-19 °C
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