14-773-M
BTK (E41K) Protein, active, 10 µg
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About This Item
Code UNSPSC :
12352204
eCl@ss :
32160405
Nomenclature NACRES :
NA.32
Source biologique
human
Niveau de qualité
100
300
Poids mol.
Mw 78.4 kDa
Fabricant/nom de marque
Upstate®
Technique(s)
activity assay: suitable (kinase)
Numéro d'accès NCBI
Numéro d'accès UniProt
Description générale
Expressed by baculovirus in Sf21 insect cells
N-terminal His6-tagged, recombinant, full-length, human BTK
Specific mutations within the pleckstrin homology (PH) domain of BTK results in human X-linked agammaglobulinemia (XLA). The E41K substitution, identified through random mutagenesis, is a PH domain gain-of-function mutation that has been shown to be associated with increased membrane localization and tyrosine phosphorylation of BTK. Using animal models, this mutation has been shown to arrest the development of immature B cells and to act as a tumour suppressor in B-cell linker protein (BLNK/SLP-65)-deficient hosts. (Li, .et al., Immunity,(1995);2:451-460 and Baraldi, E., et al., Structure,(1999);7:449-460).
Application
Active, N-terminal His6-tagged, recombinant, full-length, human BTK, for use in Kinase Assays.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Actions biochimiques/physiologiques
- Protein Target: BTK
- Target Sub-Family:TK
Conditionnement
Also available in 250 μg size --call for pricing and availability and reference catalog number 14-773M when ordering the 250 μg size.
Qualité
Routinely evaluated by phosphorylation of LKBtide.
Forme physique
Format: Ni2+/NTA-agarose
Stockage et stabilité
Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediat
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediat
Autres remarques
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Skin Sens. 1
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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