14-743-M
GCK Protein, active, 10 µg
Active, N-terminal GST-tagged, recombinant, human GCK amino acids 1-473, expressed by baculovirus in Sf21 cells. For use in Kinase Assays.
Synonyme(s) :
germinal center kinase
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About This Item
Code UNSPSC :
12352202
eCl@ss :
32160405
Nomenclature NACRES :
NA.32
Produits recommandés
Source biologique
human
Niveau de qualité
Produit recombinant
expressed in baculovirus infected Sf21 cells
Activité spécifique
2337 U/mg
Poids mol.
Mw 80.6 kDa
Fabricant/nom de marque
Upstate®
Technique(s)
activity assay: suitable (kinase)
Numéro d'accès NCBI
Numéro d'accès UniProt
Description générale
N-terminal GST- tagged, recombinant, human GCK amino acids 1-473, expressed by baculovirus in Sf21 cells.
Product Source: Recombinant protein expressed in Sf21 cells
Application
Research Category
Metabolism
Metabolism
Research Sub Category
Cardiovascular Disease
Metabolic Disorders
Cardiovascular Disease
Metabolic Disorders
Actions biochimiques/physiologiques
Protein Target: GCK
Target Sub-Family: STE
Qualité
Routinely evaluated by phosphorylation of 0.8 mg/mL myelin basic protein.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of active GCK.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of active GCK.
Définition de l'unité
Specific Activity: where one unit of GCK activity is defined as 1nmol phosphate incorporated into 0.8 mg/mL myelin basic protein per minute at 30°C with a final ATP concentration of 100 μM.
Forme physique
Purified using glutathione-agarose. Purity 52% by SDS-PAGE and Coomassie blue staining.
Stockage et stabilité
Stable for 6 months at -70 °C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 °C.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 °C.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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