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1.17081

Sigma-Aldrich

Eosin Y solution 1%, alcoholic

for microscopy

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About This Item

Code UNSPSC :
41116124
Nomenclature NACRES :
NA.41

Niveau de qualité

400

Forme

liquid

IVD

for in vitro diagnostic use

Température de transition

flash point 19 °C

Densité

0.892 g/cm3 at 20 °C

Application(s)

clinical testing
diagnostic assay manufacturing
hematology
histology

Température de stockage

15-25°C

Catégories apparentées

Description générale

Eosin Y solution 1%, alcoholic - for microscopy is used for human-medical cell diagnosis and serves the histological and clinico-cytological investigation of sample material of human origin in histological and clinico-cytological specimen materials, for example histological sections of e. g. the kidney, muscle tissue, heart, or lung. The hematoxylin and eosin (H&E) staining method is the method most frequently used for the staining of histology material. The staining mechanism behind the H&E staining is a physico-chemical process. In the first step, the positively charged nuclear dye (hematoxylin) binds to the negatively charged phosphate groups of the nucleic acid of the cell nucleus. The nuclei will be dyed dark blue to dark violet. The second step is the counterstaining with negatively charged anionic Eosin Y, a xanthene dye. Eosin binds to the positively charged plasma proteins. Cytoplasma and intercellular substances are stained pink to red, while erythrocytes will appear with yellow to orange color.
One 500ml bottle of the stain provides 1250 stainings. For more details, please see instructions for use (IFU). This product is registered as IVD and CE marked.

Remarque sur l'analyse

Suitability for microscopy (Tissue section): passes test
Nuclei: dark blue to dark violet
Cytoplasm: pink to red
intercellular substances: pink to red
Erythrocytes: yellow to orange

Pictogrammes

FlameExclamation mark
GHS02,GHS07

Mention d'avertissement

Danger

Mentions de danger

H225 - H317 - H319

Classification des risques

Eye Irrit. 2 - Flam. Liq. 2 - Skin Sens. 1

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

66.2 °F

Point d'éclair (°C)

19 °C

Certificats d'analyse (COA)

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