1.04133

Safranine O (C.I. 50240)

• Formule empirique (notation de Hill): C₂₀H₁₉ClN₄
• Numéro CAS: 477-73-6
• Poids moléculaire : 350.84
• Code UNSPSC : 41116130
• Nomenclature NACRES : NA.21

Propriétés

• Forme: solid
• Niveau de qualité: 200
• Composition: Dye, ≥80% spectrophotometric assay
• pH: 10 (20 °C, 10 g/L in H₂O)
• Solubilité: 50 g/L
• Masse volumique apparente: 400 kg/m³
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: 2-30°C
• InChI: 1S/C20H18N4.ClH/c1-12-10-17-19(11-16(12)22)24(14-6-4-3-5-7-14)18-9-8-15(21)13(2)20(18)23-17;/h3-11,22H,21H2,1-2H3;1H
• Clé InChI: QRYAEWIQIBAZOJ-UHFFFAOYSA-N

Description

Description générale

Safranin O (C.I. 50240) for microscopy Certistain^®, is a dry dye that is used for the preparation of a staining solution for e.g. Gram stain procedures in smears and sections of human origin in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Gram-negative bacteria will be counterstained by Gram′s safranin O solution and appear orange. As a CERTISTAIN^® dye it is chemically quality-certified according to highest standards and strict specifications.

Application

for microscopy Certistain^®

Remarque sur l'analyse

Dye content (spectrophotometrically): ≥ 80 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 530 - 534 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; ethanol 50 %): 1260 - 1570
TLC-Test: passes test
Loss on drying (110 °C): ≤ 15 %
Suitability for microscopy: passes test

Informations légales

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS05
• Mention d'avertissement: Danger
• Mentions de danger: H318
• Conseils de prudence: P280 - P305 + P351 + P338
• Classification des risques: Eye Dam. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3