07-449-AF647

Anti-trimethyl Histone H3 (Lys27), Alexa Fluor^™ 647 Conjugate Antibody

0.5 mg/mL, from rabbit

• Synonyme(s) : Histone H3.1t, H3/t, H3t, H3/g
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Conjugué: ALEXA FLUOR^™ 647
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): mouse (based on 100% sequence homology)
• Concentration: 0.5 mg/mL
• Technique(s): immunocytochemistry: suitable
• Numéro d'accès NCBI: NM_003493
• Numéro d'accès UniProt: Q16695
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: trimethylation (Lys27)
• Informations sur le gène: human ... H3C1(8350) ; mouse ... H3C1(360198)

Description

Description générale

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Di- and tri-methylation of histone H3 are gene silencing marks, while removal of these marks by the demethylase genes Jmjd1a and Jmjd2c leads to gene activation. This product is a conjugated version of Cat. No. 07-449.

Immunogène

Linear peptide corresponding to Histone H3 trimethylated at Lys27.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

This Anti-trimethyl Histone H3 (Lys27) antibody is validated for use in ICC for the detection of trimethyl Histone H3 (Lys27).

Qualité

Evaluated by Immunocytochemistry Analysis: in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl Histone H3 (Lys27) in HeLa cells.

Alexa Fluor^™ is a registered trademark of Life Technologies.

Description de la cible

~17 kDa observed (Refer to Cat. No. 07-449)

Forme physique

Protein A purified

Purified rabbit conjugate antibody in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Informations légales

ALEXA FLUOR is a trademark of Life Technologies

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable