07-2101-M

Anti-phospho PIX Antibody (Ser340)

from rabbit, purified by affinity chromatography

• Synonyme(s) : Rho guanine nucleotide exchange factor (GEF) 7, PAK-interacting exchange factor beta, guanine nucleotide exchange factor 7, rho guanine nucleotide exchange factor 7, SH3 domain-containing proline-rich protein, p85, PAK3, Beta-Pix, P85COOL1, COOL-1, P85,
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: mouse
• Réactivité de l'espèce (prédite par homologie): rat (based on 100% sequence homology), human (based on 100% sequence homology)
• Technique(s): western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NP_001106989
• Numéro d'accès UniProt: Q9ES28
• Modification post-traductionnelle de la cible: phosphorylation (pSer340)
• Informations sur le gène: human ... PAK3(5063)

Description

Description générale

p-21-activated kinase (PAK) interacting exchange factor (PIX) is a guanine nucleotide exchange factor. PIX is a necessary component for the recruitment, regulation and activation of Rac1, as it is able to directly bind Rac1 via its SH3 domain. Studies indicate that PIX may also have an active role as a positive regulator of apoptosis, an inducer of membrane ruffling and in cell migration. Along with Rac1, PIX has been shown to regulate and interact with GIT1 and NOD2. Alternative splicing and initiation give rise to 8 known isoforms. Expression of PIX has been localized to the central nervous system. Isoforms B, C and E have highest expression in the testis and brain.

Spécificité

This antibody recognizes PIX phosphorylated at Ser340.

Immunogène

Epitope: Phosphorylated Ser340

KLH-conjugated linear peptide corresponding to mouse PIX phosphorylated at Ser340.

Application

This phospho PIX antibody is validated for use in WB for the detection of the phospho PIX protein.

Qualité

Evaluated by Western Blot in PIX transfected cell lysates.

Western Blot Analysis: A 1:1000 dilution of this antibody detected PIX on 10 µg of Pix transfected cell lysates.

Description de la cible

~ 75 kDa

Forme physique

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide, 1 mg/mL BSA and 30% glycerol.

Remarque sur l'analyse

Control
PIX transfected cell lysates

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1