06-578-I

Anti-PI4-Kinase β

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: Unknown, polyclonal
• Poids mol.: calculated mol wt 91.38 kDa; observed mol wt ~92 kDa
• Produit purifié par: affinity chromatography
• Espèces réactives: human, monkey
• Réactivité de l'espèce (prédite par homologie): bovine
• Conditionnement: antibody small pack of 100 μL
• Technique(s): immunocytochemistry: suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: N-terminal
• Numéro d'accès Protein ID: NP_001317650
• Numéro d'accès UniProt: Q9UBF8
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: rabbit ... PI4KB(5298)

Description

Description générale

Phosphatidylinositol 4-kinase beta (UniProt: Q9UBF8; also known as EC:2.7.1.67, PI4K-beta, PI4Kbeta, PtdIns 4-kinase beta, NPIK, PI4K92, PI4KIII) is encoded by the PI4KB (also known as PIK4CB) gene (Gene ID: 5298) in human. A group of four enzymes make up the mammalian phosphatidylinositol (PI) 4-kinase family are divided into two groups based on primary sequence and biochemical properties: consisting of type II (PI4KII and PI4KIIβ) and the type III (PI4KIII and PI4KIIIβ) isozymes. They all catalyze the phosphorylation of phosphatidylinositol (PI) at the D4 position of the inositol head-group to synthesize phosphatidylinositol 4-phosphate (PI4P) that serves as an essential precursor to produce PIP2 and PIP3. PI4-kinase b is a type III peripheral membrane protein that is present in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. It phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). It also regulates Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation and is involved in Golgi-to-plasma membrane trafficking. PI4-kinase b is shown to be critical for glucose-induced insulin secretion due to its capacity to regulate the release of secretory granules. In addition, it inhibits the insulin-stimulated translocation of glucose transporter-4 in adipocytes through its interaction with neuronal calcium sensor. (Ref.: Chalupska, D., et al. (2019). Sci. Rep. 9; article 567; Clyton, EL., et al. (2013). Prog. Lipid Res. 52(3); 294-304).

Spécificité

This rabbit polyclonal antibody specifically detects Phosphatidylinositol 4-kinase b (PI4-kinase b). It targets an epitope within 18 amino acids from the N-terminal region.

Immunogène

KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of human Phosphatidylinositol 4-kinase b (PI4-Kinase b).

Application

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PI4-Kinase β in Jurkat cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PI4-Kinase β in HeLa and SH-SY5Y cell lysates.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected PI4-Kinase β in THP-1 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-PI4-Kinase β , Cat. No. 06-578-I, is a rabbit polyclonal antibody that detects Phosphatidylinositol 4-kinase b and is tested for use in Immunocytochemistry and Western Blotting.

Forme physique

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable