05-1317

Anti-SUZ12 Antibody, clone 2AO9

clone 2AO9, from mouse

• Synonyme(s) : ChET 9 protein, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein, Suppressor of zeste 12 protein homolog, joined to JAZF1, suppressor of zeste 12 homolog (Drosophila)
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 2AO9, monoclonal
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): rabbit (based on 100% sequence homology)
• Technique(s): ChIP: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable
• Isotype: IgG
• Numéro d'accès NCBI: NP_056170.2
• Numéro d'accès UniProt: Q15022
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SUZ12(23512)

Description

Description générale

SUZ12 is a polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility. This protein is component of the PRC2 complex, which methylates ′Lys-9′ and ′Lys-27′ residues of histone H3.

Spécificité

This antibody recognizes SUZ12.

Immunogène

Epitope: Unknown

Recombinant protein corresponding to human SUZ12.

Application

Immunocytochemistry Analysis: A previous lot was used by an independent laboratory in IC. Pasini, D., et al. (2007). Molecular and Cellular Biology. 27(10):3769–3779.

Chromatin Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in ChiP. Pasini, D., et al. (2007). Molecular and Cellular Biology. 27(10):3769–3779.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Use Anti-SUZ12 Antibody, clone 2AO9 (Mouse Monoclonal Antibody) validated in IP, ICC to detect SUZ12 also known as ChET 9 protein, Chromatin precipitated E2F target 9 protein, Joined to JAZF1 protein.

Qualité

Evaluated by Immunoprecipitation in HeLa cell lysate.

Immunoprecipitation Analysis: 10 µg/mL of this antibody immunoprecipitated SUZ12 in HeLa cells.

Description de la cible

~ 95 kDa

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
HeLa cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable