05-1086

Anti-phospho-IRS1 (Ser302 mouse/ Ser307 human) Antibody, clone 8.1.2

clone 8.1.2, from mouse

• Synonyme(s) : insulin receptor substrate 1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 8.1.2, monoclonal
• Espèces réactives: bovine, pig, canine, monkey, human, rat, mouse
• Technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_005535.1
• Numéro d'accès UniProt: P35568
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: phosphorylation (pSer302/pSer307)
• Informations sur le gène: bovine ... Irs1(538598) ; dog ... Irs1(486148) ; human ... IRS1(3667) ; mouse ... Irs1(16367) ; pig ... Irs1(100512686) ; rat ... Irs1(25467) ; rhesus monkey ... Irs1(707870)

Description

Description générale

IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. IRS1 is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer. Some of the more notable phosphorylation sites are Ser302 that is phosphorylated following insulin stimulation. Ser307, phosphorylated by JNK and IKK, is a key regulatory site that appears to disrupt the IRS1/IR interaction and inhibits insulin-mediated activation of the PI3 kinase and MAPK pathways, and Ser636/639 that is known to be phosphorylated by p70S6K downstream of mTOR and acts as a negative feedback loop.

Spécificité

Predicted to cross-react with many other species based on 100% sequence homology with immunogen.

This antibody recognizes IRS1 phosphorylated at Ser302 (mouse) and Ser307 (human).

Immunogène

Epitope: Phosphorylated Ser302

Synthetic linear peptide corresponding to amino acids at and around phosphorylated Ser302 of mouse IRS1.

Application

Anti-phospho-IRS1 (Ser302 mouse/ Ser307 human) Antibody, clone 8.1.2 is an antibody against phospho-IRS1 (Ser302 mouse/ Ser307 human) for use in IH, IF, IP & WB.

Immunocytochemistry:
IR/IRS transfected CHO cells were stained with a 1:500 dilution of the antibody. A seconday anti-mouse Cy3 conjugate was used for visualizaion.

Immunoprecipition:
This antibody was used at a 1:1,000 to immunoprecipitate IRS1 from 5L/mL IR/IRS1 transfected CHO cells (performed by an independent lab).

Research Category
Metabolism

Signaling

Research Sub Category
Insulin/Energy Signaling

Glucose/Glycogen Metabolism

Metabolic Hormones & Receptors

Qualité

Evaluated by western blot on IRS1 transfected CHO +/- Insulin lysate.

Western Blot Analysis:
1:1,000 dilution of this antibody was used to detect IRS1 phosphorylated on Ser302 in IRS/IR transfected CHO -/+ Calyculin A/ Okadaic Acid-treated cell lysate.

Description de la cible

~185 kDa

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8ºC from date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable