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04-293

Sigma-Aldrich

Anti-phospho-erbB2 (Thr686) Antibody, clone 7F8

clone 7F8, Upstate®, from mouse

Synonyme(s) :

NEU, NGL, HER2, TKR1, HER-2, c-erb B2

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

7F8, monoclonal

Espèces réactives

mouse, human, canine

Fabricant/nom de marque

Upstate®

Technique(s)

ELISA: suitable
western blot: suitable

Isotype

IgG1

Numéro d'accès NCBI

NM_001005862.1
NM_004448.2

Numéro d'accès UniProt

P04626

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

phosphorylation (pThr686)

Informations sur le gène

human ... ERBB2(2064)
mouse ... Erbb2(13866)

Spécificité

Recognizes phosphorylated erbB2 on Threonine 686

Immunogène

KLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 686 in human erbB2

Application

Anti-phospho-erbB2 (Thr686) Antibody, clone 7F8 is an antibody against phospho-erbB2 (Thr686) for use in ELISA & WB.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors

Kinases & Phosphatases

Qualité

Routinely evaluated by immunoblot.

Description de la cible

180 kDa

Forme physique

Format: Purified
In 1 mL of PBS containing 0.09% sodium azide, PEG, sucrose and 50% glycerol.
Subsequent thiophilic adsorption and size exclusion chromatography

Stockage et stabilité

2 years at -20°C from date of shipment. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Remarque sur l'analyse

Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

X Ouyang et al.
Carcinogenesis, 19(11), 2013-2019 (1998-12-17)
Tumour cell growth may be accelerated by protein kinase C (PKC) agonists such as phorbol esters and receptor tyrosine kinases, but receptor tyrosine kinases are in turn desensitized to growth factors by PKC agonists. To clarify this apparent PKC bifunctionality
Human cancer cells exhibit protein kinase C-dependent c-erbB-2 transmodulation that correlates with phosphatase sensitivity and kinase activity
Ouyang, X, et al
The Journal of Biological Chemistry, 271, 21786-21792 (1996)
Natasha M Bourgeois et al.
Frontiers in cellular and infection microbiology, 14, 1264525-1264525 (2024-04-08)
Dengue is an arboviral disease causing severe illness in over 500,000 people each year. Currently, there is no way to constrain dengue in the clinic. Host kinase regulators of dengue virus (DENV) infection have the potential to be disrupted by

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