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56822

Sigma-Aldrich

Immersion oil

for microscopy

Synonyme(s) :

immersion medium for light microscopy

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About This Item

Numéro MDL:
MFCD00131369
Code UNSPSC :
12171500
Nomenclature NACRES :
NA.47

Source biologique

synthetic

Qualité

for microscopy

Forme

liquid

Indice de réfraction

n20/D 1.516

Viscosité

100-120 mPa.s(20 °C)

Densité

1.025 g/mL at 20 °C

Application(s)

hematology
histology

Température de stockage

room temp

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Description générale

Immersion oil is a clear, viscous liquid with optimized refractive properties, specifically modified to closely approximate the refractive index (RI) of glass (ne = 1.5). It is used in conjunction with an objective lens to enhance the resolving power.

Application

Immersion oil is used for high-resolution (1000X) light microscopy work in conjunction with an oil immersion objective lens to optimize microscopic examinations of histological, cytological, hematological, and bacterial specimen material after it has been fixed, embedded, stained, or counterstained, and mounted.

Principe

Immersion oil is applied dropwise to stained and mounted or non-mounted specimen material to form a clear film between the specimen and the microscope lens to eliminate the deflection of incident light and thus substantially enhance the optical efficiency of the lens.

Pictogrammes

Environment
GHS09

Mention d'avertissement

Warning

Mentions de danger

H410

Conseils de prudence

P273 - P391 - P501

Classification des risques

Aquatic Acute 1 - Aquatic Chronic 2

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

Certificats d'analyse (COA)

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