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  • Vanillin Prevents Doxorubicin-Induced Apoptosis and Oxidative Stress in Rat H9c2 Cardiomyocytes.

Vanillin Prevents Doxorubicin-Induced Apoptosis and Oxidative Stress in Rat H9c2 Cardiomyocytes.

Nutrients (2020-08-06)
Ivana Sirangelo, Luigi Sapio, Angela Ragone, Silvio Naviglio, Clara Iannuzzi, Daniela Barone, Antonio Giordano, Margherita Borriello
ABSTRACT

Doxorubicin (doxo) is an effective anticancer compound in several tumor types. However, as a consequence of oxidative stress induction and ROS overproduction, its high cardiotoxicity demands urgent attention. Vanillin possesses antioxidant, antiproliferative, antidepressant and anti-glycating properties. Therefore, we investigated the potential vanillin protective effects against doxo-induced cardiotoxicity in H9c2 cells. Using multiparametric approach, we demonstrated that vanillin restored both cell viability and damage in response to doxo exposure. Contextually, vanillin decreased sub-G1 appearance and caspase-3 and PARP1 activation, reducing the doxo-related apoptosis induction. From a mechanistic point of view, vanillin hindered doxo-induced ROS accumulation and impaired the ERK phosphorylation. Notably, besides the cardioprotective effects, vanillin did not counteract the doxo effectiveness in osteosarcoma cells. Taken together, our results suggest that vanillin ameliorates doxo-induced toxicity in H9c2 cells, opening new avenues for developing alternative therapeutic approaches to prevent the anthracycline-related cardiotoxicity and to improve the long-term outcome of antineoplastic treatment.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Monoclonal Anti-β-Actin antibody produced in mouse, clone AC-74, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Vanillin, ReagentPlus®, 99%
Sigma-Aldrich
Doxorubicin hydrochloride, 98.0-102.0% (HPLC)
Sigma-Aldrich
Pentostatin, ≥95% (HPLC)
Sigma-Aldrich
Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
Sigma-Aldrich
Phosphatase Inhibitor Cocktail 1, DMSO solution
Sigma-Aldrich
RIPA Buffer
Sigma-Aldrich
Anti-PARP (Cleaved-Asp214) antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-PARP antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Sample Buffer, Laemmli 2× Concentrate
Amersham Protran® Premium Western blotting membranes, nitrocellulose, pore size 0.45 μm, roll W × L 200 mm × 4 m, pkg of 1 ea