In most circumstannces, the product is used at a 100-fold dilution. This will inhibit the phosphatases in 5 mg of protein per mL of lysate.
P2850
Phosphatase Inhibitor Cocktail 1
DMSO solution, mixture of inhibitors
Synonym(s):
Phosphatase Inhibitor
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Product Name
Phosphatase Inhibitor Cocktail 1, DMSO solution
form
DMSO solution
Quality Level
shipped in
wet ice
storage temp.
2-8°C
General description
Phosphatase Inhibitor Cocktail 1 includes a mixture of inhibitors that will inhibit the L-isozymes of alkaline phosphatase as well as serine/threonine protein phosphatases such as PP1 and PP2A. This product contains microcystinLR, cantharidin, and (−)-p-bromotetramisole. Phosphatase Inhibitor Cocktail 1 has been replaced with P0044.
Application
Biochem/physiol Actions
Phosphatases are active regulators of a number of important biological processes. Inhibition of phosphatases by small molecules is useful to study its biological role.[4]
Quantity
Recommended usage is a 100-fold dilution.
Analysis Note
Tested on cell extracts from animal tissues such as bovine liver, human placenta, and rabbit muscle, as well as on extracts from A431 and Jurkat cell lines.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
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Articles
The use of phosphatase inhibitors is critical for all types of phosphorylation studies. Compare phosphatase inhibitor cocktails to select the best one for your research.
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At what concentration should I use Product P2850, Phosphatase Inhibitor Cocktail 1?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How stable is Product P2850, Phosphatase Inhibitor Cocktail 1? Can I freeze it?
1 answer-
This reagent, as supplied and stored at 2-8°C, will be stable for four years from the date of manufacturing. Freezing is not recommended, because it may cause precipitation of the inhibitors.
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Is Product P2850, Phosphatase Inhibitor Cocktail 1, suitable for use on yeast?
1 answer-
As far as we know, the phosphatases in mammalian cells also appear in yeast cells. Thus both cocktails, P5726 (Phosphatase cocktail II and P2850 (Phosphatase Inhibitor cocktail I), may be needed with yeast extracts.
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What inhibitors are present in Product P2850, Phosphatase Inhibitor Cocktail 1, and in what concentrations?
1 answer-
Cantharidin (Product Code C7632) inhibits protein phosphatase 2A (PP-2A). Bromotetramisole (Product Code B8132) inhibits alkaline phosphatases. Microcystin LR (Product Code M2912) inhibits protein phosphatases 1 and 2A (PP-1 and PP-2A). The concentrations of these inhibitors are considered proprietary.
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Product P2850, Phosphatase Inhibitor Cocktail 1, is solid when it arrived. Is it suitable to use?
1 answer-
Since this solution is supplied in DMSO, it may solidify (melting point of DMSO is 16-19 deg C). Simply allow it to melt before use. This will not affect the product.
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