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Supelco

SUPELCOSIL™ LC-8-DB (3 µm) HPLC Columns

L × I.D. 3.3 cm × 2.1 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

SUPELCOSIL™ LC-8-DB HPLC Column, 3 μm particle size, L × I.D. 3.3 cm × 2.1 mm

material

stainless steel column

Agency

suitable for USP L7

product line

SUPELCOSIL™

feature

endcapped

manufacturer/tradename

SUPELCOSIL™

packaging

1 ea of

extent of labeling

6.0% carbon loading

parameter

0-70 °C temperature
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

3.3 cm × 2.1 mm

surface area

170 m2/g

surface coverage

surface coverage 3.2 μmol/m2

matrix

silica gel, spherical particle platform
fully porous particle

matrix active group

C8 (octyl) phase

particle size

3 μm

pore size

120 Å

operating pH

2-7.5

application(s)

food and beverages

separation technique

reversed phase

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General description

SUPELCOSIL LC-DB phases are specially deactivated for basic compounds. These columns provide less retention, better peak shape, and higher efficiency for organic bases than can be obtained on conventional reversed-phase columns.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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Certificates of Analysis (COA)

Lot/Batch Number
290239-06
290239-05
290239-04
290239-03
290239-02

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Jadwiga Piwowarska et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 805(1), 1-5 (2004-04-29)
A high-performance liquid chromatographic method is described for determination of lidocaine (2-(dietyloamino)-N-(2,6-dimetylofenylo) acetamid) and its metabolite, monoethylglycine xylidide (MEGX), in human serum containing various concentration of bilirubin. Lidocaine and its metabolite were extracted from human serum using dichloromethane. After separation
P K Kunicki
Journal of chromatography. B, Biomedical sciences and applications, 750(1), 41-49 (2001-02-24)
A HPLC-UV determination of clobazam and N-desmethylclobazam in human serum and urine is presented. After simple liquid-liquid extraction with dichloromethane the compounds and an internal standard diazepam were separated on a Supelcosil LC-8-DB column at ambient temperature under isocratic conditions
J H Nichols et al.
Clinical chemistry, 40(7 Pt 1), 1312-1316 (1994-07-01)
This automated assay determines the concentration of the antidepressant fluoxetine (Prozac) and its active metabolite norfluoxetine in serum by reversed-phase HPLC with spectrophotometric detection. Extraction, injection, and quantification are performed by the Gilson Aspec automated sample handler. The patient's specimen
Vesna Cerkvenik-Flajs et al.
Analytica chimica acta, 663(2), 165-171 (2010-03-09)
An analytical method has been developed for the simultaneous determination of the following endectocide drugs in milk: ivermectin, abamectin, doramectin, moxidectin, eprinomectin, emamectin and nemadectin. Samples were extracted with acetonitrile, purified with solid-phase extraction on a reversed phase C(8), derivatised
K Ary et al.
Journal of pharmaceutical and biomedical analysis, 26(2), 179-187 (2001-07-27)
A reversed-phase high-performance liquid chromatographic method with coulometric and UV detection has been developed for the simultaneous determination of morphine, morphine-3-glucuronide and morphine-6-glucuronide. The separation was carried out by using a Supelcosil LC-8 DB reversed-phase column and 0.1 M potassium
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