ZRB2100

Anti-MFF Antibody, clone 1E18 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Mitochondrial fission factor
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1E18, recombinant monoclonal
• description: 1E18 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 38.46 kDa; observed mol wt ~Target molecular weight 28 and 34 kDa
• purified by: using Protein A
• species reactivity: human, mouse
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal half
• Protein ID accession no.: NP_001263990
• UniProt accession no.: Q9GZY8
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... MFF(56947)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1E18 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Mitochondrial fission factor (MFF). It targets an epitope within 25 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal half of human Mitochondrial fission factor (MFF).

Application

Quality Control Testing

Evaluated by Western Blotting in T47D cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MFF in T47D cell lysates.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MFF in lysates from Human Brain and MOLT4 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MFF in human cerebral cortex tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound MFF peptide with a KD of 8.8 x 10-8 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MFF in C2C12 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Mitochondrial fission factor (UniProt: Q9GZY8; also known as MFF) is encoded by the MFF (also known as C2orf33, AD030, AD033, GL004) gene (Gene ID: 56947) in human. MFF is a single-pass type IV homodimeric membrane protein that has a cytoplasmic domain (aa 1-322), a helical domain (aa 323-340), and a mitochondrial intermembrane domain (aa 341-342). It is highly expressed in heart, kidney, liver, brain, muscle, and stomach. It is a homodimeric protein but may also interact with other proteins. It plays a role in mitochondrial and peroxisomal fission and promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. It is also suggested to be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles. MFF is shown to be an essential factor for mitochondrial recruitment of Drp1 during mitochondrial fission in mammalian cells and its down-regulation inhibits mitochondrial recruitment of Drp1 and induces mitochondria elongation. Exogenous expression of MFF shown to induce extensive mitochondrial fission. Abnormal activation of mitochondrial fission may drive the impairment of mitochondrial metabolic function that leads to inhibition of cancer stem cell propagation, and the activation of quiescence programs. Mutations in MFF gene can cause a form of encephalopathy that is characterized by delayed psychomotor development, severe hypotonia with inability to walk, and microcephaly. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Sanchez-Alvarez, R., et al. (2020). Front. Oncol. 10: 1776; Otera, H., et al. (2010). J. Cell Biol. 191 (6); 1141 1158; Gandre-Babbe, S., and van der Bliek, AM. (2008). Mol. Biol. Cell. 19(6): 2402-2412).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable