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TOX8

Sigma-Aldrich

In Vitro Toxicology Assay Kit, Resazurin based

Synonym(s):

Fluorescent resazurin toxicology assay

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1 KIT
€301.00

€301.00

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1 KIT
€301.00

About This Item

EC Number:
263-718-5
UNSPSC Code:
12352207
NACRES:
NA.84

€301.00

In StockDetails

form

liquid

Quality Level

200

usage

1 kit sufficient for 2,000 tests

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

protein quantification: suitable

application(s)

cell analysis
detection

detection method

colorimetric
fluorometric

storage temp.

2-8°C

General description

In Vitro Toxicology Assay Kit (Resazurin based) is designed for fluorometrically or spectrophotometrically determining cell number as a function of metabolic activity using the dye resazurin. The resazurin method is simple, accurate, and reproducible

Application

In Vitro Toxicology Assay Kit, Resazurin based has been used:
  • in cell viability assay of human primary fibroblasts[1]
  • to determine the cell proliferation rate in murine pre-osteoblasts[2]
  • to determine the metabolic activity of microfluidic and monolayer cultures[3]

Biochem/physiol Actions

Bioreduction of the dye reduces the amount of its oxidized form (blue) and concomitantly increases the fluorescent intermediate (red).

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Description
Pricing

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000338679
0000338679
0000277441
SLCP0774
0000277441

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José Manuel Quesada-Gómez et al.
The Journal of steroid biochemistry and molecular biology, 148, 214-218 (2014-12-03)
The nitrogen-containing or nitrogenous bisphosphonates (N-BF) are currently the main class of drugs used for the treatment of diseases characterized by an increased bone resorption. Preliminary data suggest that N-BF have also direct or indirect anti-tumoral effects, and recent evidence
A comparative study of methods for assessing the toxicity to bacteria of single chemicals and mixtures.
King, E.F. et al.
Toxicity Screening Procedures Using Bacterial Systems, 175-194 (1984)
Monika Marędziak et al.
Stem cells international, 2016, 2152435-2152435 (2016-03-05)
Tissue regeneration using human adipose derived mesenchymal stem cells (hASCs) has significant potential as a novel treatment for many degenerative bone and joint diseases. Previous studies have established that age negatively affects the proliferation status and the osteogenic and chondrogenic
A rapid biochemical test for measuring chemical toxicity.
D Liu
Bulletin of environmental contamination and toxicology, 26(2), 145-149 (1981-02-01)
Comparison of several microbiological toxicity screening tests.
Dutka, B.J., et al.
Water Research, 17, 1363-1367 (1983)
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Quality control guidelines to maintain high quality authenticated and contamination-free cell cultures. Free ECACC handbook download.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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