As mentioned on the Product Detail Page at the DESCRIPTION section, under 'Preparation Note' , this product is prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.
T8154
Trypan Blue solution
0.4%, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
Blue cell viability dye
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About This Item
Empirical Formula (Hill Notation):
C34H24N6Na4O14S4
CAS Number:
Molecular Weight:
960.81
Beilstein:
4360496
MDL number:
UNSPSC Code:
12352207
PubChem Substance ID:
NACRES:
NA.75
Pricing and availability is not currently available.
Recommended Products
sterility
sterile-filtered
Quality Level
form
liquid
storage condition
dry at room temperature
concentration
0.4%
technique(s)
cell culture | mammalian: suitable
tissue processing: suitable
application(s)
cell analysis
shipped in
ambient
SMILES string
[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
InChI
1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
InChI key
GLNADSQYFUSGOU-UHFFFAOYSA-J
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General description
For the same product in a convenient lyophilized (powder) format, please see T6146
Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue.[1] It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells.[2]
Trypan Blue (TB), an anionic hydrophilic azo dye, crosses only the cell membranes of dead cells, thereby staining dead tissues/cells blue.[1] It is used in quantitative microscopy for a dye exclusion staining technique to differentiate between live and dead mammalian cells.[2]
Trypan blue is a dye used to distinguish between live and dead cells. It is a vital stain that is not absorbed by healthy viable cells, but stains cells with a damaged cell membrane.[3] This way, only dead cells can be counted. The method is sometimes referred to as the dye exclusion method.
Application
Trypan Blue solution has been used:
- in cell viability assay to count viable and dead cells[4]
- in rescue assay to count viable cells[4]
- in trypan blue exclusion counting method to determine proliferation curve[5]
- as the saline control and in the preparation of lipopolysaccharide (LPS) solution to confirm the success of LPS infusion[6]
- in trypan blue dye exclusion test to determine gastric epithelial cells (GEC) viability[7]
- to count GEC cells using a hemocytometer[7]
Preparation Note
Prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.
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Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Carc. 1B
Storage Class Code
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
EU REACH Annex XVII (Restriction List)
CAS No.
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is this solution dissolved in PBS?
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What is the stability of the product. How long we can use it as there is no expiry date mentioned in the COA
1 answer-
This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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Why is Trypan blue, Product T8154, used to determine cell viability?
1 answer-
Trypan Blue is a vital stain recommended for use in estimating the proportion of viable cells in a population. The reactivity of this dye is based on the fact that the chromophore is negatively charged and does not react with the cell unless the membrane is damaged. Staining facilitates the visualization of cell morphology. Live (viable) cells do not take up the dye and dead (non-viable) cells do.
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Do the cells need to be washed to remove the culture media prior to staining with Product T8154, Trypan Blue solution?
1 answer-
Trypan Blue has a greater affinity for serum proteins than for cellular protein. If the background is too dark, cells should be pelleted and resuspended in protein-free medium or salt solution prior to counting.
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Can Trypan blue, Product T8154, be used to discriminate between apoptotic and necrotic cells?
1 answer-
Trypan blue will stain cells that have disrupted membranes. It cannot differentiate between permeabilized membranes caused by apoptosis or necrosis.
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Trypan blue, Product T8154, contains a precipitate. Is it still good to use?
1 answer-
The trypan blue solution is still good to use. It can be warmed to dissolve the precipitate or it can be filtered to remove the precipitate.
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Why are there red fibers in Product T8154, Trypan blue solution?
1 answer-
A report of fibers in Product T8154, Trypan blue solution is sufficiently common almost to be a characteristic of the product. These fibers are a cosmetic fault rather than one of functionality. The presence of fibers may be more pronounced following cold storage or shipping. This product should be stored at room temperature. Filtration to remove the fibers will not affect the use of the product.
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